Droplet generator

A droplet generator, comprising a wrapped biological material flow channel, an encoded microsphere flow channel and an oil-phase flow channel;

• wherein the encoded microsphere flow channel comprises an arc-shaped flow channel and two straight flow channels, the arc-shaped flow channel comprises one or more segments of circular arc-shaped flow channels with different radii, at least one of the circular arc-shaped flow channels has a radius that is more than 6 times the width of the encoded microsphere flow channel;
• wherein, the encoded microsphere flow channel is also provided with an encoded microsphere flow channel tank, the flow resistance of the encoded microsphere fluid in the encoded microsphere buffer tank is less than the flow resistance of the encoded microsphere flow channel;
• the biological material can be selected from one or more of cells, nucleic acids, proteins, antibodies or antibody fragments;
• wherein extension lines of the two straight flow channels that are connected with the arc-shaped flow channel intersect at an acute angle;
• wherein the straight flow channels connecting the two arc-shaped flow channels are arranged to be inclined upwardly, the arc-shaped flow channel and the straight flow channel that is connected with the arc-shaped flow channel form a large-corner continuous U-shaped flow channel of the encoded microsphere flow channel;
• wherein the shape of the encoded microsphere buffer tank gradually enlarges from top to bottom, and then rapidly retracts downwardly.