Back to results
US 12,599,667 B2Granted
Methods of preventing platelet alloimmunization and alloimmune platelet refractoriness and induction of tolerance in transfused recipients
AssigneeBloodworks
InventorSherrill J. Slichter
Life of this patent
Estimating the term…
Abstract
Methods and compositions for the prevention or reduction of platelet transfusion associated complications are provided. The subject methods include modifying donor whole blood or platelets prior to transfusion to prevent or reduce alloimmune platelet refractoriness.
Claims15
A method for reducing risk of platelet alloimmunization upon transfusion, the method comprising:
- filtering platelet rich plasma (PRP) from a donor through a leukoreduction filter;
- performing pathogen reduction on the PRP by adding a photosensitizer compound to the PRP and irradiating the PRP and photosensitizer compound with UV light and thereby substantially eliminating donor lymphocytes from the PRP;
- γ-irradiating the filtered and pathogen-reduced PRP;
- and
- transfusing the filtered, pathogen-reduced, and γ-irradiated PRP into a recipient, wherein the transfused PRP has an acceptance rate of 90% or higher, and wherein following the transfusion, the recipient is antibody negative to donor lymphocytes;
- thereby
- reducing the risk of the recipient developing platelet alloimmunization upon receiving transfused donor platelets.
4 more elements
A method of preventing platelet refractoriness in a recipient receiving platelets from an antigenically mismatched donor, the method comprising:
- filtering whole blood from a donor through a leukoreduction filter;
- performing pathogen reduction on the whole blood by adding a photosensitizer to the whole blood and irradiating the whole blood and the photosensitizer with UV light and thereby substantially eliminating donor lymphocytes from the whole blood;
- and
- γ-irradiating the filtered and pathogen-reduced whole blood;
- and
- transfusing the γ-irradiated, filtered, and pathogen-reduced whole blood into the recipient, wherein the transfused whole blood has an acceptance rate of 90% or higher, wherein following the transfusion the recipient is antibody negative to donor lymphocytes, and wherein the transfused whole blood do not cause the recipient to develop platelet refractoriness.
3 more elements
Dependencies are inferred from the claim text.