# US 12,599,642 B2 · Compositions derived from Salvia hispanica seeds

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- Number: US 12,599,642 B2
- Document ID: US12599642B2
- Status: Granted
- Kind: B2
- Application number: 17/909,387
- Filed: 2021-03-04
- Published: 2023-04-13
- Granted: 2026-04-14
- Earliest claimed: 2020-03-04
- Assignees: Signum Biosciences, Inc.
- Applicants: Signum Biosciences
- Inventors: Jose Fernandez; Eddie Perez; Kristen Huber; Jason Healy; Corey Webb; Karl Rouzard; Mike Voronkov; George Nikonov
- Web page: https://patentgrep.com/patent/US12599642B2

## CPC

- A61K 9/0014: {Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00)}
- A61K 36/537: Salvia (sage)
- A61K 8/9789: Magnoliopsida [dicotyledons]
- A61P 17/00: Drugs for dermatological disorders
- A61P 17/06: Antipsoriatics
- A61P 25/00: Drugs for disorders of the nervous system
- A61P 25/28: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- A61P 31/04: Antibacterial agents
- A61Q 17/04: Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
- A61Q 19/00: Preparations for care of the skin
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K 2236/15: involving mechanical treatment, e.g. chopping up, cutting or grinding
- A61K 2236/33: involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K 2236/37: Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
- A61K 2236/51: Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
- A61K 2236/53: Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

Methods of preparing a composition from chia seeds are provided. Compositions, including compositions prepared by the presently disclosed methods, and methods regarding selecting chia seeds and administering chia seed extracts are also disclosed.

## Claims

### Claim 1

A method of preparing an extract from chia seeds, said method comprising:
a) introducing acetonitrile to chia seeds to form a mixture;
b) agitating the mixture at a maintained temperature above room temperature for a period of time;
c) filtering the chia seeds from the agitated mixture;
d) evaporating the filtered mixture under reduced pressure to remove the acetonitrile and to form a crude chia seed oil;
e) introducing methyl t-butyl ether to the crude chia seed oil to form a solution;
f) adding a decolorant to the solution;
g) agitating the solution containing the decolorant for a second period of time;
h) filtering the decolorant from the agitated solution; and
i) evaporating the methyl t-butyl ether from the filtered solution under reduced pressure, thereby preparing the extract,
wherein relative to the crude chia seed oil, the extract comprises one or more of the following:
an increased amount of cis, cis-9, 12-octadecadienoic acid (LA);
an increased amount of cis, cis, cis-9, 12, 15-octadecatrienoic acid (ALA); and/or
increased stability and reduced color change when stored at 50° C. for up to eight weeks.

### Claim 2 (depends on 1)

The method of claim 1, wherein the ratio of the acetonitrile-to-chia seed is from about 2:1 to about 4:1 (w/v).

### Claim 3 (depends on 2)

The method of claim 2, wherein the ratio of the acetonitrile-to-chia seed is about 3:1 (w/v).

### Claim 4 (depends on 1)

The method of claim 1, wherein the mixture is agitated for at least 12 hours and the maintained temperature is at least 40° C.

### Claim 5 (depends on 1)

The method of claim 1, wherein the mixture is evaporated under reduced pressure with a rotary evaporator to form the crude chia seed oil.

### Claim 6 (depends on 1)

The method of claim 1, wherein the methyl t-butyl ether is removed using vacuum evaporation at room temperature.

### Claim 7 (depends on 1)

The method of claim 1, wherein the decolorant is an absorbent.

### Claim 8 (depends on 7)

The method of claim 7, wherein the absorbent is selected from the group consisting of activated carbon, silica gel, decolorizing clay and activated alumina.

### Claim 9 (depends on 8)

The method of claim 8, wherein the absorbent is fuller's earth decolorizing clay.

### Claim 10 (depends on 8)

The method of claim 8, wherein the absorbent is activated carbon.

### Claim 11 (depends on 1)

The method of claim 1, wherein the solution is agitated with the decolorant for at least 8 hours.

### Claim 12 (depends on 1)

The method of claim 1, wherein the decolorant is filtered from the solution.

### Claim 13 (depends on 12)

The method of claim 12, wherein the decolorant is filtered from the solution using at least two filters.

### Claim 14 (depends on 1)

The method of claim 1, wherein the absorbent is filtered from the solution serially with at least two filters.

### Claim 15 (depends on 14)

The method of claim 14, wherein at least one of the filters is a diatomaceous earth filter.

### Claim 16 (depends on 14)

The method of claim 14, wherein at least one of the filters is a silica gel filter.

### Claim 17 (depends on 1)

The method of claim 1, wherein the solution is evaporated under reduced pressure with a rotary evaporator.

### Claim 18 (depends on 1)

The method of claim 1, further comprising grinding the chia seeds prior to introducing the acetonitrile.