# US 12,599,629 B2 · Coagulation mold, a kit and a method for preparing a coagulated blood mass

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- Number: US 12,599,629 B2
- Document ID: US12599629B2
- Status: Granted
- Kind: B2
- Application number: 19/032,762
- Filed: 2025-01-21
- Published: 2025-05-22
- Granted: 2026-04-14
- Earliest claimed: 2022-01-25
- Assignees: Reddress Ltd.
- Applicants: Reddress Ltd.
- Inventors: Alon Kushnir
- Web page: https://patentgrep.com/patent/US12599629B2

## CPC

- A61K 35/14: Blood; Artificial blood (perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42)
- A61K 47/02: Inorganic compounds
- A61K 47/14: Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
- A61P 7/00: Drugs for disorders of the blood or the extracellular fluid
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present disclosure provides a method, a coagulation mold and a kit for preparing a coagulated blood mass having a desired shape and volume. The coagulated blood mass is prepared from whole blood being withdrawn from a subject. The whole blood is mixed with coagulation initiators in order to obtain the coagulated blood mass with optimal parameters. This is obtained, inter alia, by selecting particle size of one of the coagulation initiators such as to yield more rapid dissolving within the blood and/or to yield improved suspension of the particles in the blood when it is still in a liquid form. By controlling theses parameters, an optimal coagulated blood mass is obtained that can be used for treatment of the human body, e.g. for treating skin lesions, internal injuries, such as anal fistula, stoma cavity, damaged tendon, and others.

## Claims

### Claim 1

A method for preparing a coagulated blood mass, suitable for use in a treatment of a subject, comprising the steps of:
mixing an amount of whole blood with calcium gluconate powder and kaolin powder; and
allowing the whole blood to coagulate to form the coagulated blood mass;
wherein
an amount of calcium gluconate powder mixed with whole blood is about 3.3 mg-7.3 mg per 1 ml of whole blood; and an amount of kaolin powder mixed with whole blood is about 0.6 mg-3.1 mg per 1 ml of whole blood.

### Claim 2 (depends on 1)

The method of claim 1, wherein at least one of the following applies: (i) the particle-size distribution D50 of the calcium gluconate powder is 50μ-200μ; or (ii) the particle-size distribution D50 of the kaolin powder is less than 25μ.

### Claim 3 (depends on 1)

The method of claim 1, wherein the particle-size distribution D90 of the calcium gluconate powder is between 50μ-200μ.

### Claim 4 (depends on 1)

The method of claim 1, wherein the particle-size distribution D99 of the kaolin powder is less than 50μ.

### Claim 5 (depends on 1)

The method of claim 1, wherein the particle-size distribution D80 of the kaolin powder is less than 25μ.

### Claim 6 (depends on 1)

The method of claim 1, wherein the amount of the kaolin powder mixed with the whole blood being 0.6 mg-3.1 mg per 1 ml of whole blood.

### Claim 7 (depends on 6)

The method of claim 6, wherein the amount of the kaolin powder mixed with the whole blood being about 1.75 mg per 1 ml of whole blood.

### Claim 8 (depends on 1)

The method of claim 1, wherein the amount of calcium gluconate powder mixed with the whole blood is about 5.3 mg per 1 ml of whole blood.

### Claim 9 (depends on 1)

The method of claim 1, wherein said mixing is performed within a coagulation mold.