Method of obtaining a pharmaceutical agent used for inhibiting the proliferation of tumor cells

Abstract

The given invention is related to the field of medicine, namely to experimental research in oncology, and can be used to obtain a pharmaceutical agent for inhibiting the proliferation of HeLa cervical cancer cells. It entails the use of a metal powder obtained in a gaseous medium by means of an electric explosion of a wire made of low-carbon steel carried out with a specific energy of 7-18 KJ/g and a pulse duration of 1.2-2 μs. The explosion is carried out inside a reactor that is pre-evacuated to a residual pressure of 10−2 Pa. and then filled with carbon monoxide to a pressure of 105 Pa at a gas circulation rate flow of 10 m/s. The products of the explosion deposited in a hopper are passivated in air for at least 48 hours. The resulting powder is extracted and mixed with a solution of the RPMI-1640 nutrient medium with L-glutamine, a pH of 7.2, in the proportion of the mass of the powder to the volume of the specified solution from 1:10 to 2.7:10. The solution is then centrifuged until the phases are separated. The liquid phase is decanted and used as a pharmaceutical agent. The purpose of the given invention is to expand the arsenal of tools for inhibiting the proliferation of tumor cells.

Dependencies are inferred from the claim text.