# US 12,599,573 B2 · Compositions and uses thereof in treating cancers

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- Number: US 12,599,573 B2
- Document ID: US12599573B2
- Status: Granted
- Kind: B2
- Application number: 18/185,630
- Filed: 2023-03-17
- Published: 2024-09-19
- Granted: 2026-04-14
- Earliest claimed: 2022-03-17
- Assignees: National Cheng Kung University
- Applicants: National Cheng Kung University
- Inventors: Yan-Shen Shan; Chang-Jung Chen; Hao-Chen Wang
- Web page: https://patentgrep.com/patent/US12599573B2

## CPC

- A61K 9/5123: {Organic compounds, e.g. fats, sugars}
- A61K 9/0019: {Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00)}
- A61K 9/1271: Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers (liposomes as conjugates {A61K47/6911})
- A61K 9/5146: {obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides}
- A61K 31/713: Double-stranded nucleic acids or oligonucleotides
- A61P 35/00: Antineoplastic agents
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Disclosed herein is a composition comprising a lipid nanoparticle and a double-stranded oligodeoxynucleotide (dsODN) encapsulated in the lipid nanoparticle. According to the embodiments of the present disclosure, the dsODN comprises two strands complementary to each other, in which the first strand comprises the nucleotide sequence of SEQ ID NO: 1. Also disclosed herein are methods of treating cancers by using the present composition.

## Claims

### Claim 1

A composition comprising,
a double-stranded oligodeoxynucleotide (dsODN) that inhibits binding of signal transducer and activator of transcription 6 (STAT6) and histone deacetylase 2 (HDAC2) to cytochrome b-245 beta chain (CYBB) promotor, the dsODN comprises a first strand having a nucleotide sequence 100% identical to SEQ ID NO:3, and a second strand complementary to the first strand; and
a lipid nanoparticle having a hydrophilic core and an outer lipid bilayer;
wherein,
the dsODN is encapsulated within the hydrophilic core of the lipid nanoparticle.

### Claim 2 (depends on 1)

A method of treating a cancer in a subject, comprising administering to the subject an effective amount of the composition of claim 1.

### Claim 3 (depends on 2)

The method of claim 2, wherein the cancer is gastric cancer, lung cancer, bladder cancer, breast cancer, pancreatic cancer, renal cancer, colorectal cancer, cervical cancer, ovarian cancer, brain tumor, prostate cancer, hepatocellular carcinoma, melanoma, esophageal carcinoma, multiple myeloma, or head and neck squamous cell carcinoma.

### Claim 4 (depends on 2)

The method of claim 2, wherein the subject is a human.