Methods and systems for producing skin grafts
AssigneeCutiss AG
InventorsVincent Ronfard, Claude Holenstein, Anna-Lena Dittrich, Laurent Barnes, Reto Frei, Ulrich Öffinger, Boris Stolz
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Abstract
Various methods for producing skin grafts for rehabilitation of skin defects are carried out in a closed system operated and controlled in an automated manner. A skin biopsy may be separated into a dermis layer and an epidermis layer prior to formation of a skin graft. A total skin graft area produced may be 9 times to 1000 times greater than a total skin biopsy area that provides cells for the graft. Differences in density between dermis and epidermis may aid in separation between a dermis layer and an epidermis layer.
Claims9
A method for producing a skin graft, comprising:
- connecting a biopsy kit containing a skin biopsy sample to a layer separation chamber;
- enzymatically separating the skin biopsy into a dense dermis layer and a less dense epidermis layer in said layer separation chamber, and transferring the dense dermis layer by flushing through a first outlet, and transferring the less dense epidermis layer by flushing through a second outlet, the separation chamber being assisted by external agitation;
- isolating single cell suspension of fibroblasts and keratinocytes from the separated dermis and epidermis layers of the biopsy sample respectively, and transferring the isolated fibroblasts to one chamber and the isolated keratinocytes to a second chamber;
- expanding fibroblasts and keratinocytes in their respective first and second chambers, and transferring the expanded fibroblasts and keratinocytes to first and second reservoirs respectively;
- processing the fibroblasts and keratinocytes in their respective reservoirs;
- and
- forming a skin graft comprising fibroblasts and keratinocytes from the processed fibroblasts and keratinocytes;
- wherein connecting, separating, isolating, expanding, processing, and forming are subject to the automation of the process.
A method comprising:
- enzymatically separating a skin biopsy into a dense dermis layer and a less dense epidermis layer in a chamber at least partially filled with liquid;
- separating the dense dermis layer from the less dense epidermis layer by flushing the dermis layer through a first outlet and flushing the epidermis layer through a second outlet situated above said first outlet with the assistance of external agitation;
- isolating a single cell suspension of fibroblasts from the dermis layer and a single cell suspension of keratinocytes from the epidermis layer, and
- transferring the isolated fibroblasts to one chamber and the isolated keratinocytes to a second chamber.
Dependencies are inferred from the claim text.