# US 12,599,070 B2 · Genetic loci associated with disease resistance in soybeans

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- Number: US 12,599,070 B2
- Document ID: US12599070B2
- Status: Granted
- Kind: B2
- Application number: 17/631,324
- Filed: 2020-07-31
- Published: 2022-10-27
- Granted: 2026-04-14
- Earliest claimed: 2019-07-31
- Assignees: Syngenta Crop Protection AG
- Applicants: Syngenta Crop Protection AG
- Inventors: Qingli Liu; Robert Arthur Dietrich; Thomas Joseph Curley, Jr.; John Daniel Hipskind; Becky Welsh Breitinger; John Luther Dawson
- Web page: https://patentgrep.com/patent/US12599070B2

## CPC

- A01H 1/045: {using molecular markers}
- A01H 1/1255: {for fungal resistance}
- A01H 5/10: Seeds
- A01H 6/54: Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
- A01H 6/542: {Glycine max [soybean]}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using markers, genes and chromosomal intervals derived from Glycine clandestina, e.g. PI339656, or a progeny thereof. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.

## Claims

### Claim 1

A method of selecting a soybean plant having an Asian Soy Rust (ASR) resistance allele, the method comprising the steps of:
a. Isolating a nucleic acid from a soybean plant;
b. Detecting in the nucleic acid the presence of a molecular marker that associates with an ASR resistance allele, wherein the molecular marker is a T at a position corresponding to position 3,275,655 of SEQ ID NO: 1, or a T at a position corresponding to position 3,274,672 of SEQ ID NO: 1, or a T at a position corresponding to position 3,291,351 of SEQ ID NO: 1;
c. thereby identifying a soybean plant having an ASR resistance allele; and
d. selecting the soybean plant identified at step c as having the ASR resistance allele.

### Claim 2 (depends on 1)

The method of claim 1, wherein the ASR resistance allele is a T at position 3,274,672 of SEQ ID NO: 1.

### Claim 3 (depends on 1)

The method of claim 1, wherein the ASR resistance allele is a T at position 3,291,351 of SEQ ID NO: 1.