Digital synthesis of histological stains using multiplexed immunofluorescence imaging

A system comprising:

• one or more data processors;
• and
• a non-transitory computer readable storage medium containing instructions which, when executed on the one or more data processors, cause the one or more data processors to perform a set of actions including:
• producing an N-channel input image that is based on information from each of M channels of a multiplexed immunofluorescence (MPX) image of a tissue section, where M is a positive integer and N is a positive integer that is less than or equal to M;
• and
• generating a synthetic image by processing the N-channel input image using a generator network, the generator network having been trained using a training data set that includes a plurality of pairs of images,
• wherein the synthetic image depicts a tissue section that has been stained with at least one histochemical stain, and
• wherein, for each pair of images of the plurality of pairs of images, the pair includes:
• an N-channel image produced from an MPX image of a first section of a tissue, and
• an image of a second section of the tissue that has been stained with the at least one histochemical stain.

A computer-program product tangibly embodied in a non-transitory machine-readable storage medium, including instructions configured to cause one or more data processors to perform a set of actions including:

• producing an N-channel input image that is based on information from each of M channels of a multiplexed immunofluorescence (MPX) image of a tissue section, where M is a positive integer and N is a positive integer that is less than or equal to M;
• and
• generating a synthetic image by processing the N-channel input image using a generator network, the generator network having been trained using a training data set that includes a plurality of pairs of images,
• wherein the synthetic image depicts a tissue section that has been stained with at least one histochemical stain, and
• wherein, for each pair of images of the plurality of pairs of images, the pair includes:
• an N-channel image produced from an MPX image of a first section of a tissue, and
• an image of a second section of the tissue that has been stained with the at least one histochemical stain.