US 12,596,123 B2Granted

Information processing apparatus, biological specimen analysis method, biological specimen detection apparatus, and biological specimen detection system

AssigneeSony Group Corporation

InventorsTomohiko Nakamura, Kazuhiro Nakagawa

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Abstract

To provide a technique for supporting selection of a binding molecule and a labeled molecule to be used in detecting and/or analyzing a target molecule. An information processing apparatus, including: a processing unit configured to calculate, based on a signal derived from a sample including a biological specimen, when using a plurality of different binding molecules labeled by different labeled molecules, reactivity between a target molecule and each of the plurality of different binding molecules, wherein the signal includes: a first signal group acquired when each of a plurality of different binding molecules having been labeled by a labeled molecule of a same type is reacted with a target molecule, and a second signal group acquired when each of binding molecules of a same type having been labeled by different labeled molecules is reacted with a target molecule.

Claims15

Dependent claims

An information processing apparatus, comprising:

A processor configured to calculate, based on a signal derived from a sample including a biological specimen, when using a plurality of different binding molecules labeled by different labeled molecules, reactivity between a target molecule and each of the plurality of different binding molecules, wherein
the signal includes:
a first signal group acquired when each of a plurality of different binding molecules having been labeled by a labeled molecule of a same type is reacted with the target molecule, and
a second signal group acquired when each of a binding molecule of a same type having been labeled by different labeled molecules is reacted with the target molecule;
and
wherein the processor is configured to select a combination of the labeled molecule and the binding molecule by one or more methods selected from (a) to (f) below:
(a) select the combination of the labeled molecule and the binding molecule of which a specific signal/background equals or exceeds a threshold;
(b) select the combination of the labeled molecule and the binding molecule which maximizes a sum of a specific signal/background;
(c) select the combination of the labeled molecule and the binding molecule which maximizes a sum of differences between a signal of fluorescence intensity and a background of the fluorescence intensity;
(d) select the combination of the labeled molecule and the binding molecule based on a magnitude of a signal of fluorescence intensity;
(e) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in a descending order of the signal of fluorescence intensity to binding molecules in an ascending order of a value of the first signal group;
and
(f) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in an ascending order of a length of a detection wavelength to binding molecules in a descending order of a value of the first signal group;
and
wherein in each of the one or more selected methods from (a) to (f), the significance of the binding molecule and/or the labeled molecule with respect to the target molecule is estimated by determining whether or not to raise a luminance in accordance with a type of the binding molecule or the labeled molecule, considering how the threshold is to be set, and lowering a background to enable an object signal to be picked up more easily when the object signal is weak.

12 more elements

A biological specimen analysis method, comprising the steps of:

acquiring a signal derived from a sample including a biological specimen;
calculating, based on the signal, when using a plurality of different binding molecules labeled by different labeled molecules, reactivity between a target molecule and each of the plurality of different binding molecules;
and outputting the reactivity, wherein
the signal includes:
a first signal group acquired when each of a plurality of different binding molecules having been labeled by a labeled molecule of a same type is reacted with the target molecule, and
a second signal group acquired when each of a binding molecule of a same type having been labeled by different labeled molecules is reacted with the target molecule;
and
wherein the processor is configured to select a combination of the labeled molecule and the binding molecule by one or more methods selected from (a) to (f) below:
(a) select the combination of the labeled molecule and the binding molecule of which a specific signal/background equals or exceeds a threshold;
(b) select the combination of the labeled molecule and the binding molecule which maximizes a sum of a specific signal/background;
(c) select the combination of the labeled molecule and the binding molecule which maximizes a sum of differences between a signal of fluorescence intensity and a background of the fluorescence intensity;
(d) select the combination of the labeled molecule and the binding molecule based on a magnitude of a signal of fluorescence intensity;
(e) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in a descending order of the signal of fluorescence intensity to binding molecules in an ascending order of a value of the first signal group;
and
(f) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in an ascending order of a length of a detection wavelength to binding molecules in a descending order of a value of the first signal group;
and
wherein in each of the one or more selected methods from (a) to (f), the significance of the binding molecule and/or the labeled molecule with respect to the target molecule is estimated by determining whether or not to raise a luminance in accordance with a type of the binding molecule or the labeled molecule, considering how the threshold is to be set, and lowering a background to enable an object signal to be picked up more easily when the object signal is weak.

14 more elements

A biological specimen detection apparatus, comprising:

A sample signal acquirer configured to acquire a signal derived from a sample including a biological specimen;
an information processing apparatus including:
a processor configured to calculate, based on the signal, when using a plurality of different binding molecules labeled by different labeled molecules, reactivity between a target molecule and each of the plurality of different binding molecules;
a detection process output router configured to output the reactivity;
and
a signal detector configured to detect a signal emitted from the target molecule labeled using a binding molecule which is selected based on the output reactivity and which is labeled by a labeled molecule, wherein the signal includes:
a first signal group acquired when each of a plurality of different binding molecules having been labeled by a labeled molecule of a same type is reacted with the target molecule, and
a second signal group acquired when each of the binding molecules of a same type having been labeled by different labeled molecules is reacted with the target molecule;
and
wherein the processor is configured to select a combination of the labeled molecule and the binding molecule by one or more methods selected from (a) to (f) below:
(a) select the combination of the labeled molecule and the binding molecule of which a specific signal/background equals or exceeds a threshold;
(b) select the combination of the labeled molecule and the binding molecule which maximizes a sum of a specific signal/background;
(c) select the combination of the labeled molecule and the binding molecule which maximizes a sum of differences between a signal of fluorescence intensity and a background of the fluorescence intensity;
(d) select the combination of the labeled molecule and the binding molecule based on a magnitude of a signal of fluorescence intensity;
(e) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in a descending order of the signal of fluorescence intensity to binding molecules in an ascending order of a value of the first signal group;
and
(f) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in an ascending order of a length of a detection wavelength to binding molecules in a descending order of a value of the first signal group;
and
wherein in each of the one or more selected methods from (a) to (f), the significance of the binding molecule and/or the labeled molecule with respect to the target molecule is estimated by determining whether or not to raise a luminance in accordance with a type of the binding molecule or the labeled molecule, considering how the threshold is to be set, and lowering a background to enable an object signal to be picked up more easily when the object signal is weak.

16 more elements

A biological specimen detection system, comprising:

an information processing apparatus including:
a sample signal acquirer configured to acquire a signal derived from a sample including a biological specimen;
a processor configured to calculate, based on the signal, when using a plurality of different binding molecules labeled by different labeled molecules, reactivity between a target molecule and each of the plurality of different binding molecules;
a detection process output router configured to output the reactivity, the signal including:
a first signal group acquired when each of a plurality of different binding molecules having been labeled by a labeled molecule of a same type is reacted with the target molecule, and
a second signal group acquired when each of a binding molecule of a same type having been labeled by different labeled molecules is reacted with the target molecule;
and
wherein the processor is configured to select a combination of the labeled molecule and the binding molecule by one or more methods selected from (a) to (f) below:
(a) select the combination of the labeled molecule and the binding molecule of which a specific signal/background equals or exceeds a threshold;
(b) select the combination of the labeled molecule and the binding molecule which maximizes a sum of a specific signal/background;
(c) select the combination of the labeled molecule and the binding molecule which maximizes a sum of differences between a signal of fluorescence intensity and a background of the fluorescence intensity;
(d) select the combination of the labeled molecule and the binding molecule based on a magnitude of a signal of fluorescence intensity;
(e) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in a descending order of the signal of fluorescence intensity to binding molecules in an ascending order of a value of the first signal group;
and
(f) select the combination of the labeled molecule and the binding molecule so that labels are to be assigned in an ascending order of a length of a detection wavelength to binding molecules in a descending order of a value of the first signal group;
and
wherein in each of the one or more selected methods from (a) to (f), the significance of the binding molecule and/or the labeled molecule with respect to the target molecule is estimated by determining whether or not to raise a luminance in accordance with a type of the binding molecule or the labeled molecule, considering how the threshold is to be set, and lowering a background to enable an object signal to be picked up more easily when the object signal is weak, and
a detection apparatus configured to detect the signal emitted from the target molecule labeled using the binding molecule which is selected based on the output reactivity and which is labeled by the labeled molecule.

15 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/927,333Filed 28 May 2021
Priority: JP 2020-09473329 May 2020
Published: US 2023/0288427 A114 Sep 2023
Granted: 7 Apr 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 33/5302
Immunoassay; Biospecific binding assay; Materials therefor: apparatus specially adapted for immunological test procedures
G01N 33/582
Involving labelled substances: with fluorescent label
B01L 3/502715
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
G01N 21/6428
Fluorescence; Phosphorescence: measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g.…