Means and methods for detecting soy allergens

A method for the manufacture of an antibody that specifically binds to soy Gly m8 protein wherein said method comprises:

• a) immunizing an animal with purified soy Gly m8 protein, wherein said soy Gly m8 protein has been obtained by:
• i) transiently expressing soy Gly m8 protein in plants;
• and
• ii) purifying the soy Gly m8 protein from said plants;
• and
• b) obtaining an antibody from the animal which specifically binds to soy Gly m8 protein,
• wherein the obtaining an antibody from the animal comprises generating hybridoma clones from the animal and selecting and cultivating a hybridoma clone that produces an antibody that specifically binds to soy Gly m8 protein, wherein the antibody has a KD of at least 1.57×10−10 for the soy Gly m8 protein, and wherein the antibody is selected from the group consisting of mAb3 produced by hybridoma mouse anti-Gly m 8-3 deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under Accession Number DSM ACC3383 and mAb8 produced by hybridoma mouse anti-Gly m 8-8 deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) under Accession Number DSM ACC3384.