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US 12,596,108 B2Granted
Automated lipid exchange-mass spectrometry systems and methods
AssigneeArizona Board of Regents on Behalf of the University of Arizona
InventorsMichael Marty, James Keener, Guozhi Zhang
Life of this patent
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Abstract
An automated system for lipid exchange-mass spectrometry, e.g., measuring affinity of a membrane protein for lipids. The automated systems herein can measure the specificity of membrane protein-lipid interactions, detect remodeling of the membrane environment, and determine optimal lipid composition for membrane proteins.
Claims20
A method for measuring affinity of a membrane protein for lipids, said method comprising:
- a) incubating untagged lipid nanoparticles with a population of tagged lipid nanoparticles, wherein the untagged lipid nanoparticles comprise one or more lipids and a membrane protein, wherein the membrane protein is embedded in the untagged lipid nanoparticles, wherein the tagged lipid nanoparticles comprise one or more lipids without the membrane protein embedded therein;
- b) separating the tagged lipid nanoparticles from the untagged lipid nanoparticles;
- c) measuring a lipid composition of the untagged lipid nanoparticles;
- and
- d) calculating a difference between the lipid composition of the untagged lipid nanoparticles and a lipid composition of the combination of both tagged lipid nanoparticles and untagged lipid nanoparticles;
- wherein one or more of steps (a)-(d) are automated.
3 more elements
Dependencies are inferred from the claim text.