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US 12,595,592 B2Granted
Comprehensive monoclonal antibody generation
AssigneeBioAtla, Inc.
InventorJay M. Short
Life of this patent
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Abstract
The present invention relates to methods for efficiently generating recombinant monoclonal antibodies derived from B cells of a non-human host which has been immunochallenged with one or more target antigens. The methods comprise the steps of identifying and isolating B cell that bind to the antigen by FACS, and recombining and enriching for thousands of cells to create a B cell library. Related products and methods, such as methods of producing expression libraries, are also disclosed.
Claims32
A method of generating and identifying a recombinant antibody that binds at least one target antigen comprising:
- a) amplifying cDNA obtained from mRNA of B cells from a human or a non-human animal selected from a rabbit, a mouse, a rat, a hamster, a guinea pig, and a goat, which are capable of binding to the at least one target antigen to prepare a cDNA library comprising cDNA encoding VH and VL domains;
- b) generating antibodies from the VH and VL domains in a eukaryotic cell production host whereby the antibodies comprise light chain/heavy chain combinations;
- c) screening the antibodies with the at least one target antigen to identify a subset of antibodies capable of binding to the at least one target antigen;
- and
- d) expressing at least one of the identified subset of antibodies in the same eukaryotic cell production host as was used in the generating step, whereby the recombinant antibody that binds at least one target antigen is identified and generated.
2 more elements
Dependencies are inferred from the claim text.