Molecular markers for reduced pyruvate level trait in Allium cepa

A genotyping array comprising two or more SNPs of:

• SNP_01 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 1;
• SNP_02 comprising an Adenine at nucleotide 51 of SEQ ID NO:
• 3;
• SNP_03 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 5;
• SNP_04 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 7;
• SNP_05 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 9;
• SNP_06 comprising an Adenine at nucleotide 51 of SEQ ID NO:
• 11;
• SNP_07 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 13;
• SNP_08 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 15;
• SNP_09 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 17;
• SNP_10 comprising a Guanine at nucleotide 51 of SEQ ID NO:
• 19;
• SNP_11 comprising an Adenine at nucleotide 51 of SEQ ID NO:
• 21;
• SNP_12 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 23;
• SNP_13 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 25;
• SNP_14 comprising an Adenine at nucleotide 51 of SEQ ID NO:
• 27;
• SNP_15 comprising a Guanine at nucleotide 51 of SEQ ID NO:
• 29;
• SNP_16 comprising an Adenine at nucleotide 51 of SEQ ID NO:
• 31;
• SNP_17 comprising an Adenine at nucleotide 51 of SEQ ID NO:
• 33;
• SNP_18 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 35;
• SNP_19 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 37;
• SNP_20 comprising a Guanine at nucleotide 51 of SEQ ID NO:
• 39;
• SNP_21 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 41;
• SNP_22 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 43;
• SNP_23 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 45;
• SNP_24 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 47;
• and/or
• SNP_25 comprising a Guanine at nucleotide 51 of SEQ ID NO:
• 49.

A method for producing an Allium cepa plant or plant part comprising a Quantitative Trait Loci (QTL) conferring a reduced pyruvate level when compared to an Allium cepa plant or plant part not comprising said QTL, said method comprising:

• crossing an Allium cepa plant comprising said QTL conferring a reduced pyruvate level with another Allium cepa plant, and
• selecting for offspring from the crossing comprising one or more markers linked to a reduced pyruvate conferring QTL located on chromosome 1 flanked by the markers SNP_05 comprising a Cytosine at nucleotide 51 of SEQ ID NO:
• 9 and SNP_08 comprising a Thymine at nucleotide 51 of SEQ ID NO:
• 15 by selecting said one or more markers based on marker assisted selection;
• wherein said QTL conferring a reduced pyruvate level is as present in a plant of which seeds were deposited under Accession No. PTA-9053, a plant of which seeds were deposited under Accession No. PTA-9054 or a plant of which seeds were deposited under Accession No. PTA-9055.