Methods of producing omega-hydroxylated fatty acid derivatives
AssigneeGenomatica, Inc.
InventorsAndreas W. Schirmer, Haibo Wang, Stephen B. Del Cardayre, Zhihao Hu, Louis G. Hom, Baolong Zhu, Cindy Chang, Emanuela E. Popova
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Abstract
The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.
Claims21
A modified ω-hydroxylase of EC 1.14.15.3, comprising a modified CYP153A polypeptide having at least 90% sequence identity to SEQ ID NO:4, wherein the modified ω-hydroxylase comprises at least one mutation at an amino acid position corresponding to one or more of positions 6, 11, 27, 40, 41, 82, 116, 129, 131, 132, 134, 136, 138, 141, 142, 144, 149, 153, 154, 157, 161, 168, 178, 205, 228, 231, 233, 258, 271, 304, 308, 309, 407, 415, and 419 of SEQ ID NO:4.
A CYP153A-reductase hybrid fusion polypeptide variant, comprising at least 90% sequence identity to SEQ ID NO:6 and comprising at least one mutation at an amino acid position corresponding to one or more of positions 6, 11, 27, 40, 41, 82, 116, 129, 131, 132, 134, 136, 138, 141, 142, 144, 149, 153, 154, 157, 161, 168, 178, 205, 228, 231, 233, 258, 271, 304, 308, 309, 407, 415, 419, 516, 666, 696, and 796, wherein the CYP153A-reductase hybrid fusion polypeptide variant catalyzes the conversion of a fatty acid or derivative thereof to an ω-hydroxy fatty acid or derivative thereof.
Dependencies are inferred from the claim text.