# US 12,595,484 B2 · Composition for regulating production of interfering ribonucleic acid

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- Number: US 12,595,484 B2
- Document ID: US12595484B2
- Status: Granted
- Kind: B2
- Application number: 18/933,819
- Filed: 2024-10-31
- Published: 2025-05-22
- Granted: 2026-04-07
- Earliest claimed: 2023-11-22
- Assignees: Wyvern Pharmaceuticals Inc.
- Applicants: Wyvern Pharmaceuticals Inc.
- Inventors: Bradley G. Thompson
- Web page: https://patentgrep.com/patent/US12595484B2

## CPC

- C12N 15/111: {General methods applicable to biologically active non-coding nucleic acids}
- C12N 15/1136: {against growth factors, growth regulators, cytokines, lymphokines or hormones}
- C12N 15/86: Viral vectors
- C12N 2310/14: interfering nucleic acids [NA]
- C12N 2310/141: MicroRNAs, miRNAs
- C12N 2330/51: Specially adapted vectors
- C12N 2750/14141: Use of virus, viral particle or viral elements as a vector
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The embodiments of the present disclosure relate to decreasing the bioavailability of one or more target biomolecules by providing a composition that comprises a recombinant plasmid with one or more sequences of micro interfering ribonucleic acid (miRNA). When the recombinant plasmid interacts with a target cell, it causes the target cell to upregulate production of the miRNA, which then decreases the bioavailability of the target biomolecule. In some embodiments of the present disclosure, the target biomolecule is a cytokine or other mediator molecule of an inflammatory response.

## Claims

### Claim 1

A composition that comprises a recombinant plasmid (RP) comprising a sequence of nucleotides that is SEQ ID NO: 7.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the RP is encapsulated in a protein coat, a lipid vesicle, or any combination thereof.

### Claim 3

A composition that comprises a recombinant plasmid (RP) comprising a sequence of nucleotides that is SEQ ID NO: 17.

### Claim 4 (depends on 3)

The composition of claim 3, wherein the RP is encapsulated in a protein coat, a lipid vesicle, or any combination thereof.