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US 12,595,472 B2Granted
Mannanase variants
AssigneeBASF SE
InventorsKatie Kline, Amanda Rae Logue, Asfia Qureshi, Cindy Hoang, Jesper Nielsen, Mark Miller, James Garrett Bonner
Life of this patent
Estimating the term…
Abstract
A mannanase at least 75% identical to SEQ ID NO: 2 or SEQ ID NO: 3, a polynucleotide encoding the mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.
Claims14
A mannanase variant comprising an amino acid sequence having at least 90% sequence identity to the full-length amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 3, comprising at least one amino acid substitution selected from N341F, F346T, T348S/R/N/M/G, E349T/S/G/D, S352N/G, G356Y/V/T/Q/H/C, and D379V, wherein the numbering is according to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.