NKR variants for increased production of isobutanol
AssigneeGevo, Inc.
InventorsChristopher Smith, Timothy Hanly, Jeanne Benoit
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Abstract
The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.
Claims10
A recombinant microorganism comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI), wherein said KARI is at least about 98% identical to SEQ ID NO:
- 90, SEQ ID NO:
- 92, or SEQ ID NO:
- 94.
Dependencies are inferred from the claim text.