Injectable off-the-shelf cartilage, tendon, and ligament repair compositions and methods of use

A method comprising:

• isolating stem cells from an allogenic source or an autologous source;
• culturing the stem cells in a chondrogenic medium until they form one or more condensed mesenchymal cell bodies (CMBs);
• freezing the CMBs at a temperature of less than about −80° C. at an immature state;
• and
• storing the CMBs, at the temperature of less than about −80° C. and at an immature state, for at least one day at an immature state, wherein a CMB at the immature state is a CMB that has been cultured for fewer than six days prior to freezing and storing at the temperature of less than about −80° C.,
• wherein the CMBs, after thawing, are configured to mature in vivo and are effective to form cartilage in a site of a tissue defect, and
• wherein the cartilage is integrated with surrounding connective tissue.