# US 12,595,442 B2 · Lipase variants and compositions comprising surfactant and lipase variant

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- Number: US 12,595,442 B2
- Document ID: US12595442B2
- Status: Granted
- Kind: B2
- Application number: 18/392,370
- Filed: 2023-12-21
- Published: 2024-04-18
- Granted: 2026-04-07
- Earliest claimed: 2016-06-30
- Assignees: Novozymes A/S
- Applicants: Novozymes A/S
- Inventors: Kim Borch; Carsten Hørslev Hansen; Jesper Vind; Marco Malten; Svend Gunnar Kaasgaard
- Web page: https://patentgrep.com/patent/US12595442B2

## CPC

- C11D 3/38627: {containing lipase}
- C11D 1/14: derived from aliphatic hydrocarbons or mono-alcohols
- C11D 1/22: derived from aromatic compounds
- C11D 1/83: Mixtures of non-ionic with anionic compounds
- C11D 3/33: Amino carboxylic acids
- C11D 3/38609: {Protease or amylase in solid compositions only}
- C11D 3/38618: {Protease or amylase in liquid compositions only}
- C11D 3/50: Perfumes
- C12N 9/20: Triglyceride splitting, e.g. by means of lipase
- C11D 1/72: Ethers of polyoxyalkylene glycols
- C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase}
- C11D 2111/12: Soft surfaces, e.g. textile
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions.

## Claims

### Claim 1

A lipase variant of a parent lipase, which (a) comprises a substitution at positions corresponding to positions 1, 210, and 233 of SEQ ID NO: 1; (b) has a sequence identity of at least 85% but less than 100% sequence identity to SEQ ID NO: 1; and (c) has lipase activity.

### Claim 2 (depends on 1)

The lipase variant according to claim 1, wherein the variant further comprises a modification in at least one of the following positions: V2, D27, N33, G38, F51, E56, L69, D96, K98, D111, G163, V176, H198, Y220, L227, T231, K237, D254, and P256 of SEQ ID NO: 1.

### Claim 3 (depends on 1)

The lipase variant according to claim 1, wherein the variant further comprises at least one of the following substitutions: V2Y, D27R, N33K, N33Q, G38A, F51V, E56K, L69R, D96E, D96L, K98I, K98Q, D111A, G163K, V176L, H198S, Y220F, L227G, T231R, N233C, K237C, D254S, and P256T of SEQ ID NO: 1.

### Claim 4 (depends on 1)

The lipase variant of claim 1, comprising E210T/N/A/Q/R.

### Claim 5 (depends on 1)

The lipase variant of claim 1, comprising N233C or N233R.

### Claim 6 (depends on 1)

The lipase variant of claim 1, comprising E1C.

### Claim 7 (depends on 1)

The lipase variant of claim 1, further comprising a modification in at least one of the following positions: V2, N33, G38, E56, L69, V176, L227, K237, and D254 of SEQ ID NO: 1.

### Claim 8 (depends on 1)

The lipase variant of claim 1, further comprising a modification selected from the following substitutions: V2Y, N33K, N33Q, G38A, E56K, L69R, V176L, L227G, K237C, and D254S.

### Claim 9 (depends on 1)

The lipase variant of claim 1, which has a sequence identity of at least 90% sequence identity to SEQ ID NO: 1.

### Claim 10 (depends on 1)

The lipase variant of claim 1, which has a sequence identity of at least 95% sequence identity to SEQ ID NO: 1.

### Claim 11 (depends on 1)

The lipase variant of claim 1, wherein the parent lipase is SEQ ID NO: 1.

### Claim 12 (depends on 1)

A composition comprising a lipase variant of claim 1 and a surfactant.

### Claim 13 (depends on 12)

The composition of claim 12, further comprising at least one additional enzyme selected from the group consisting of amylase, cellulase, beta-glucanase, mannanase, and protease.

### Claim 14 (depends on 12)

A method of laundering, comprising laundering a fabric with a composition according to claim 12 at a temperature of 50° C. or less.

### Claim 15 (depends on 12)

A method of pre-treating a fabric with a composition according to claim 12, comprising the steps of adding said composition to said fabric, and leaving the composition on the fabric for a period of time, and rinsing off said composition from said fabric.