# US 12,595,315 B2 · Monoclonal antibody of matrix metalloproteinase-1, detection kit and detection method thereof

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- Number: US 12,595,315 B2
- Document ID: US12595315B2
- Status: Granted
- Kind: B2
- Application number: 17/791,744
- Filed: 2020-02-27
- Published: 2023-06-22
- Granted: 2026-04-07
- Earliest claimed: 2020-02-27
- Assignees: S&T Biomed Co., Ltd.
- Applicants: S&T Biomed Co., Ltd.
- Inventors: Ya-Ting Chang; Jau-Song Yu; Jun-Sheng Wang; Shu-Fang Wu; Chih-Ju Chen; Yen-Chun Liu
- Web page: https://patentgrep.com/patent/US12595315B2

## CPC

- C07K 16/40: against enzymes
- C07K 16/28: against receptors, cell surface antigens or cell surface determinants
- G01N 33/54387: {Immunochromatographic test strips}
- G01N 33/54388: {based on lateral flow}
- C07K 2317/565: Complementarity determining region [CDR]
- G01N 2333/96494: Matrix metalloproteases, e. g. 3.4.24.7
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Provided is a monoclonal antibody of matrix metalloproteinase 1. The monoclonal antibody has a heavy chain variable region with an amino sequence comprising i) CDR1 selected from the group consisting of SEQ ID NOs: 1, 7 and 13, ii) CDR2 selected from the group consisting of SEQ ID NOs: 2, 8 and 14, and iii) CDR3 selected from the group consisting of SEQ ID NOs: 3, 9 and 15. The monoclonal antibody also has a light chain variable region with an amino sequence comprising i) CDR1 selected from the group consisting of SEQ ID NOs: 4, 10 and 16, ii) CDR2 selected from the group consisting of SEQ ID NOS: 5, 11 and 17, and iii) CDR3 selected from the group consisting of SEQ ID NOs: 6, 12 and 18. A polynucleotide, a detection kit and a detection method are also provided as well.

## Claims

### Claim 1

A monoclonal antibody, comprising:
a monoclonal antibody A, wherein a heavy chain variable region sequence of the monoclonal antibody A comprises amino acid sequences of SEQ ID NOs: 1, 2, and 3, and a light chain variable region sequence of the monoclonal antibody A comprises amino acid sequences of SEQ ID NOs: 4, 5, and 6;
a monoclonal antibody B, wherein a heavy chain variable region sequence of the monoclonal antibody B comprises amino acid sequences of SEQ ID NOs: 7, 8, and 9, and a light chain variable region sequence of the monoclonal antibody B comprises amino acid sequences of SEQ ID NOs: 10, 11, and 12; or
a monoclonal antibody C, wherein a heavy chain variable region sequence of the monoclonal antibody C comprises amino acid sequences of SEQ ID NOs: 13, 14, and 15, and a light chain variable region sequence of the monoclonal antibody C comprises amino acid sequences of SEQ ID NOs: 16, 17, and 18.

### Claim 2 (depends on 1)

A polynucleotide, wherein the polynucleotide encodes the full length of the monoclonal antibody A, the full length of monoclonal antibody B, or the full length of the-monoclonal antibody C of claim 1, or has a sequence complementary to a nucleotide sequence that encodes the full length of the monoclonal antibody A, the full length of the-monoclonal antibody B, or the full length of monoclonal antibody C of claim 1.

### Claim 3

A detection kit, wherein the detection kit comprises a monoclonal antibody A, wherein the monoclonal antibody A comprises:
a heavy chain variable region comprising amino acid sequences of SEQ ID NOs: 1, 2, and 3; and
a light chain variable region comprising amino acid sequences of SEQ ID NOs: 4, 5, and 6.

### Claim 4 (depends on 3)

The detection kit of claim 3, wherein the detection kit further comprises an enzyme immunoassay reagent kit, a colloidal gold immunoassay test strip or a combination thereof.

### Claim 5 (depends on 4)

The detection kit of claim 4, wherein the enzyme immunoassay reagent kit comprises:
a monoclonal antibody B, wherein the monoclonal antibody B comprises:
a heavy chain variable region comprising amino acid sequences of SEQ ID NOs: 7, 8, and 9; and
a light chain variable region comprising amino acid sequences of SEQ ID NOs: 10, 11, and 12.

### Claim 6 (depends on 5)

The detection kit of claim 5, wherein the monoclonal antibody B is linked to a chromogenic group.

### Claim 7 (depends on 4)

The detection kit of claim 4, wherein the colloidal gold immunoassay test strip comprises:
a monoclonal antibody C, comprising:
a heavy chain variable region comprising amino acid sequences of SEQ ID NOs: 13, 14, and 15; and
a light chain variable region comprising amino acid sequences of SEQ ID NOs: 16, 17, and 18.

### Claim 8 (depends on 4)

The detection kit of claim 4, wherein the colloidal gold immunoassay test strip further comprises the monoclonal antibody A, and the monoclonal antibody A in the colloidal gold immunoassay test strip is linked to a gold particle.

### Claim 9 (depends on 4, 7)

A method for detecting matrix metalloproteinase-1 in vitro, comprising:
detecting matrix metalloproteinase-1 in a sample using the detection kit of claim 4,
wherein when the detection kit comprises the colloidal gold immunoassay test strip, using the detection kit of claim 7 comprises adding the sample to a colloidal gold pad containing a detection antibody-gold particle conjugate, wherein the detection antibody-gold particle conjugate comprises the monoclonal antibody A, and
wherein when the detection kit comprises the enzyme immunoassay reagent kit, using the detection kit of claim 7 comprises performing a sandwich enzyme-linked immunosorbent assay with the monoclonal antibody A as a capture antibody.

### Claim 10 (depends on 9)

The method of claim 9, wherein the sample comprises a body fluid or a blood.

### Claim 11 (depends on 10)

The method of claim 10, wherein the body fluid comprises an oral secretion or a respiratory secretion.

### Claim 12 (depends on 7)

The detection kit of claim 7, wherein the colloidal gold immunoassay test strip further comprises the monoclonal antibody A, and the monoclonal antibody A in the colloidal gold immunoassay test strip is linked to a gold particle.