US 12,594,557 B2Granted

Isoelectric focusing devices and fixtures

AssigneeIntabio, Inc.

InventorsLuc Bousse, Don Wesley Arnold, Wesley Chang, Erik Gentalen, Eric Gwerder, Scott Mack

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Abstract

Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. Two or more isoelectric focusing reactions may be performed in parallel. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

Claims17

Dependent claims

A system comprising:

a fluidic device, wherein the fluidic device comprises
at least one fluid inlet,
at least one fluid outlet,
at least one separation channel comprising a first end that is fluidically coupled to the at least one fluid inlet and a second end that is fluidically coupled to the at least one fluid outlet,
and a mobilizer channel that intersects with a second end of the separation channel, and
a nebulizer, wherein the nebulizer is configured to surround at least part of the fluidic device on at least four sides and wherein the fluidic device is polygonal,
wherein a gap between the nebulizer and the fluidic device is formed, and
wherein the nebulizer comprises a chamber and an inlet that directs nebulizing gas inside the gap.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/318,294Filed 16 May 2023 · continuation in part
Priority: 12 Aug 2019
Published: US 2023/0381781 A130 Nov 2023
Granted: 7 Apr 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification25 codes

B01L 3/502776
Characterised by multiphase flow arrangements: specially adapted for focusing or laminating flows
G01N 27/44756
Using electrophoresis: apparatus specially adapted therefor
B01L 3/5027
With fluid transport, e.g. in multi-compartment structures: by integrated microfluidic structures, i.e. dimensions of channels and chambers are…
B01L 3/502715
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…

Family

Application62/885,733Us provisional application · filed 12 Aug 2019
Application62/893,549Us provisional application · filed 29 Aug 2019
Application62/909,675Us provisional application · filed 2 Oct 2019
Application16/808,063Parent of this continuation · filed 3 Mar 2020
Application17/123,474Parent of this continuation · filed 16 Dec 2020
US 10,870,113 B1Parent of this continuation · filed 22 Dec 2020
Application17/544,250Parent of this continuation-in-part · filed 7 Dec 2021
US 2023/0381781 A1Pre-grant publication · published 30 Nov 2023
US 11,224,875 B2Parent of this continuation
Application17/544,250Parent of this continuation
US 2021/0101152 A1Published application
US 2022/0088605 A1Published application
US 12,594,557 B2This patent

From the US filing history. Foreign family members aren’t listed.