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US 12,594,556 B2Granted
Method and device for high field strength electrotransfection of microvescicles and cells
AssigneeThe Charles Stark Draper Laboratory, Inc.
InventorsJonathan R. Coppeta, Timothy J. Biliouris, Daniel F. King, Vishal Tandon
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Abstract
A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.
Claims18
An electroporation system comprising:
- an electroporation device comprising a flow channel and a pair of electrodes, the electroporation device comprising a conductive material configured to apply an electric field to the flow channel in response to a voltage applied across the pair of electrodes;
- a voltage generator electrically coupled to the pair of electrodes and configured to apply the voltage across the pair of electrodes;
- a pump fluidly coupled to the flow channel, the pump configured to direct a fluid through the flow channel and configured to generate a pressure of two atmospheres or greater in the fluid within the flow channel;
- and
- a controller operably coupled to the voltage generator and the pump, the controller configured to operate the pump to direct the fluid through the flow channel and generate the pressure in the fluid within the flow channel, the controller further configured to simultaneously operate the voltage generator to apply the voltage across the pair of electrodes to apply the electric field to the fluid while the fluid is at the pressure of two atmospheres or greater within the flow channel.
2 more elements
Dependencies are inferred from the claim text.