Hemostatic microparticles and dressing
AssigneeOceanit Laboratories, Inc.
InventorsJoanne S. M. Ebesu, Jacob Pollock
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Abstract
Disclosed herein is a method of manufacturing hemostatic particles, which involves heating and mixing a chitosan solution and a gelatin solution to form a chitosan/gelatin solution, adding the chitosan/gelatin mixture to oil containing an emulsifier/surfactant to form a combination of chitosan/gelatin and oil, heating and mixing the combination and then cooling and stirring the combination to form gelatin-chitosan microspheres (GCM), adding an aqueous solution of glutaraldehyde to the combination and stirring to cross-link the GCM, washing the GCM with emulsifier/surfactant to remove the oil, washing the GCM with a solvent to further remove excess oil, washing the GCM with alcohol to remove excess water, and drying the GCM.
Claims3
A method for manufacturing chitosan-combination microspheres, the method comprising:
- heating and mixing equal volumes of a chitosan solution and a gelatin solution, thereby generating an admixture, wherein the heating occurs at a temperature between 40° C. and 80° C.;
- adding the admixture to oil containing an emulsifier/surfactant to form a mixture;
- stirring the mixture;
- adding a 70% glutaraldehyde aqueous solution to the mixture at a volume/volume ratio of 1:2.5 of the admixture, thereby generating chitosan-combination microspheres;
- stirring the chitosan-combination microspheres;
- washing the chitosan-combination microspheres;
- and
- drying the chitosan-combination microspheres,
- wherein the chitosan-combination microspheres have a diameter of between 50 μm to 999 μm,
- wherein the washing further comprises:
- removing the oil from the chitosan-combination microspheres with a dilute polysorbate 80 solution;
- after the removing the oil, washing with acetone;
- and
- after the washing with acetone, washing with isopropyl alcohol.
Dependencies are inferred from the claim text.