Production of exosomes and uses thereof
AssigneesChina Medical University, China Medical University Hospital, Shine-On Biomedical Co., Ltd.
InventorsYi-Wen Chen, Der-Yang Cho, Hsin-Yuan Fang, Ming-You Shie, Chih-Ming Pan, Kai-Wen Kan, Cheng-Yu Chen, Min-Hua Yu, Shao-Chih Chiu
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Abstract
A method for producing exosomes in a large-scale by using a cyclic tensile bioreactor to stimulate cells to release exosomes. In addition, the exosome having an anti-HLA-G protein specific for cancer is used as a delivery vehicle to deliver therapeutic agents for treating cancer.
Claims10
A drug carrier, which comprises an exosome comprising a fusion protein, wherein the fusion protein is expressed on the membrane of the exosome and comprises a targeting protein and an exosomal transmembrane protein, the targeting protein comprises an anti-HLA-G protein, and the sequence of the anti-HLA-G protein comprises the peptide sequence of SEQ ID NO:
- 2, the exosomal transmembrane protein is connected to the targeting protein and used to express the targeting protein on the outside of the exosomal membrane, and the targeting protein is used to bind to HLA-G proteins expressed on cancer cells.
A method for treating a subject with cancer, comprising administering a composition into the subject with cancer, wherein the composition comprises a therapeutic exosome, and the therapeutic exosome comprises a fusion protein and an anti-cancer agent, and the fusion protein is expressed on the membrane of the therapeutic exosome and comprises a targeting protein and an exosomal transmembrane protein, the targeting protein comprises an anti-HLA-G protein, and the sequence of the anti-HLA-G protein comprises the peptide sequence of SEQ ID NO:
- 2, the exosomal transmembrane protein is connected to the targeting protein and used to express the targeting protein on the outside of the therapeutic exosomal membrane, and the targeting protein is used to bind to HLA-G proteins expressed on cancer cells.
Dependencies are inferred from the claim text.