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US 12,594,329 B2Granted
Virus-like particles
AssigneeUniversity of Denver
InventorSchuyler B. van Engelenburg
Life of this patent
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Abstract
Virus-like particles, compositions and antibody detection tests with virus-like particles, and a method of producing virus-like particles are disclosed. The method includes generating a modified viral genome based on a viral genome, wherein a portion of the viral genome is removed to generate the modified viral genome configured to yield virus-like particles that are unable to replicate, and producing one or more virus-like particles using the modified viral genome.
Claims9
A method for producing replication-incompetent coronavirus-like particles, the method comprising:
- cloning one-third or less of a coronavirus genome into deoxyribonucleic acid (DNA) forms comprising a first plasmid and a second plasmid, the first plasmid, in a (−) sense orientation, coding for viral structural and accessory proteins, and the second plasmid coding for a minimal replicase system;
- introducing the plasmids into cultured cells;
- transcribing the first plasmid to produce a structural transcript and transcribing the second plasmid to produce a replicase-encoding transcript;
- producing sub-genomic ribonucleic acids (RNAs) with the minimal replicase system from the first plasmid to express S, E, M, and N proteins in native-like stoichiometry;
- and
- assembling and releasing virus-like particles using the sub-genomic RNAs and translated products.
3 more elements
Dependencies are inferred from the claim text.