# US 12,593,849 B2 · Temperature-optimized bacilli > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,593,849 B2 - Document ID: US12593849B2 - Status: Granted - Kind: B2 - Application number: 17/638,152 - Filed: 2020-08-28 - Published: 2022-11-03 - Granted: 2026-04-07 - Earliest claimed: 2019-08-29 - Assignees: Chr. Hansen A/S - Applicants: Chr. Hansen A/S - Inventors: Patricia Dominguez Cuevas; Raquel Azevedo; Karin Bjerre; Iuliana Nita; Rute Neves; Lars Moelbak - Web page: https://patentgrep.com/patent/US12593849B2 ## CPC - A01N 63/22: Bacillus - C07K 14/32: from Bacillus (G) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived. ## Claims ### Claim 1 A composition comprising Bacillus paralicheniformis strain CHCC36494, deposited at Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig (DSMZ) as accession number DSM 33239 in an agrochemically acceptable carrier. ### Claim 2 (depends on 1) The composition of claim 1, comprising about 1×102 to about 1×109 colony forming units of Bacillus paralicheniformis strain CHCC36494 per gram of said composition. ### Claim 3 (depends on 1) The composition of claim 1, comprising at least 1×109 colony forming units of Bacillus paralicheniformis strain CHCC36494 per gram of said composition. ### Claim 4 (depends on 1) The composition of claim 1, further comprising one or more additional microorganisms. ### Claim 5 (depends on 1) The composition of claim 1, further comprising one or more additional strains of Bacillus. ### Claim 6 (depends on 1) The composition of claim 1, further comprising one or more strains of Bacillus subtilis. ### Claim 7 (depends on 1) The composition of claim 1, further comprising one or more chemical insecticides, fungicides, nematicides and/or bactericides. ### Claim 8 (depends on 1) The composition of claim 1, further comprising one or more plant extracts. ### Claim 9 (depends on 1) The composition of claim 1, further comprising one or more plant growth regulators. ### Claim 10 (depends on 1) The composition of claim 1, further comprising one or more fertilizers. ### Claim 11 (depends on 1) A kit comprising the composition of claim 1 and instructions for applying said composition to a plant seed, to a plant growth medium and/or to a plant. ### Claim 12 (depends on 1) A plant seed that is at least partially coated with the composition of claim 1. ### Claim 13 (depends on 12) The plant seed of claim 12, comprising about 1×102 to about 1×109 colony forming units of Bacillus paralicheniformis strain CHCC36494. ### Claim 14 (depends on 1) A kit comprising a plurality of plant seeds that are at least partially coated with the composition of claim 1 and instructions for using said plurality of seeds. ### Claim 15 (depends on 14) The kit of claim 14, comprising about 1×102 to about 1×109 colony forming units of Bacillus paralicheniformis strain CHCC36494 per seed. ### Claim 16 (depends on 1) A method comprising applying the composition of claim 1 to a plant seed. ### Claim 17 (depends on 1) A method comprising introducing the composition of claim 1 into a plant growth medium. ### Claim 18 (depends on 1) A method comprising applying the composition of claim 1 to a plant. ### Claim 19 (depends on 1) A method comprising treating a plant or plant seed with the composition of claim 1 in an amount effective to enhance growth of said plant or plant seed. ### Claim 20 (depends on 1) A method comprising treating a plant or plant seed with the composition of claim 1 in an amount effective to confer protection to said plant or plant seed against one or more plant pathogens. ### Claim 21 (depends on 20) The method of claim 20, wherein said one or more plant pathogens comprises one or more Meloidogyne, Pratylenchus, Heterodera, Globodera, Ditylenchus, Tylenchulus, Xiphinema, Radopholus, Rotylenchulus, Helicotylenchus and/or Belonolaimus.