Back to results
US 12,593,766 B2Granted
Methods for generating plants producing seeds having altered seed composition
AssigneePioneer Hi-Bred International, Inc.
InventorsJanel M Bettis, John D Everard, Kristin Haug Collet, Bo Shen, Shreedharan Sriram
Life of this patent
Estimating the term…
Abstract
Provided is a method for producing plants having seeds with an increased protein and/or oil content using mutagenesis and non-destructive chemical analysis. Additionally, provided are methods for identifying causative mutations associated with increased protein and/or oil content and methods of generating plants having the causative mutations associated with increased protein and/or oil content.
Claims20
A method for producing plants having seeds with increased protein or oil from a mutagenized population of seeds, the method comprising:
- (a) providing a mutagenized population of seeds, the mutagenized population of seeds having a genetic background modified by mutations introduced by random mutagenesis;
- (b) performing a high-throughput near infrared single-seed, non-destructive measurement of the protein or oil content of a sample of seeds comprising a subset of the mutagenized population of seeds, a corresponding control, or a combination thereof;
- (c) setting a threshold protein or oil value based upon the measurement of protein or oil content respectively of the sample of seeds, wherein the threshold protein or oil value is set above the average of the protein or oil content of the sample of seeds;
- (d) separating seeds that exceed the threshold protein or oil value from the mutagenized population of seeds;
- (e) generating plants from the separated seeds that exceed the threshold protein or oil value, wherein the plants comprise an introduced mutation resulting in increased protein or oil;
- (f) collecting seeds from individual plants produced in (e), wherein the individual plants are optionally chosen based on one or more agronomic or seed composition characteristics;
- (g) determining the average protein and/or oil content of the seeds collected from an individual plant in (f) using a second non-destructive analysis;
- (h) identifying plants that produce seeds having an average protein and/or oil content that achieves a second threshold protein value and/or a second threshold oil value, wherein the second threshold protein value is a plant average seed protein content of at least 34% by weight at 13% moisture, the second threshold oil value is a plant average seed oil content of at least 16% by weight at 13% moisture, or the second threshold oil value is both a plant average seed protein content of at least 34% by weight at 13% moisture and a plant average seed oil content of at least 16% by weight at 13% moisture;
- (i) selecting the seeds from the plants of (h) that achieve the second threshold protein value and/or the second threshold oil value;
- and
- (j) generating plants from the seed selected in (i).
8 more elements
A method for producing plants having seeds with increased oil and protein from a mutagenized population of seeds, the method comprising:
- (a) providing a mutagenized population of seeds, the mutagenized population of seeds having a genetic background modified by mutations introduced by random mutagenesis;
- (b) performing a high-throughput near infrared single-seed, non-destructive measurement of the protein and oil content of a sample of seeds comprising a subset of the mutagenized population of seeds, a corresponding control, or a combination thereof;
- (c) setting a threshold protein and oil value based upon the measurement of protein and oil content of the sample of seeds, wherein the threshold value is set above the average of the protein and oil content of the sample of seeds;
- (d) separating seeds that exceed the threshold oil and protein value from the mutagenized population of seeds;
- and
- (e) generating plants from the separated seeds that exceed the threshold protein and oil value, wherein the plants comprise an introduced mutation resulting in increased protein and oil;
- (f) collecting seeds from individual plants produced in (e), wherein the individual plants are optionally chosen based on one or more agronomic or seed composition characteristics;
- (g) determining the average protein and/or oil content of the seeds collected from an individual plant in (f) using a second non-destructive analysis;
- (h) identifying plants that produce seeds having an average protein and oil content that achieves a second threshold protein value and a second threshold oil value, wherein the second threshold protein value is a plant average seed protein content of at least 34% by weight at 13% moisture and the second threshold oil value is a plant average seed oil content of at least 16% by weight at 13% moisture;
- (i) selecting the seeds from the plants of (h) that achieve the second threshold protein value and the second threshold oil value;
- and
- (j) generating plants from the seed selected in (i).
9 more elements
Dependencies are inferred from the claim text.