Methods, devices, and systems for detecting analyte levels

A computer-implemented method, comprising:

• (a) capturing, by a camera of a mobile telecommunications device, an image of a detection region on a substrate in contact with a biological sample from a subject, wherein the biological sample is urine, wherein the detection region comprises a detector that undergoes a change in a plurality of visible properties of the detection region in response to contact with an analyte in the biological sample, wherein the analyte is associated with a hormone profile of the subject, wherein the analyte is selected from the group consisting of:
• luteinizing hormone, progesterone or a metabolite thereof, estrogen or a metabolite thereof, and human chorionic gonadotropin;
• (b) processing, by a processor of the mobile telecommunications device, pixel intensities of an RGB image of the detection region on the substrate in contact with the biological sample, wherein the processing is in real-time, wherein the processing comprises:
• processing the RGB image by a rule-based image processing algorithm, wherein the rule-based image processing algorithm processes the RGB image by:
• 1) colorspace conversion;
• 2) topological/morphological transformation;
• 3) declivity-based edge detection;
• 4) clustering using a Gaussian mixture models
• 5) normalization using adaptive histogram equalization;
• and
• 6) calibration using an information storage code,
• thereby quantifying an amount of the analyte in the detection region on the substrate to determine a concentration of the analyte in the biological sample based on a training set of quantified concentrations of a plurality of analytes, wherein the pixel intensities correspond to the plurality of visible properties of the detector, wherein the visible properties are hue, saturation, value, and lightness color space;
• c collecting, by a computing network connected to the mobile telecommunications device, the amount of the analyte;
• d analyzing, by the computing network, the amount of the analyte, thereby generating analyzed data specific to the subject;
• e generating, by the computing network, a report on the hormone profile specific to the subject based on the analyzing;
• f providing, by the processor of the mobile telecommunications device, the report on the hormone profile of the subject.