Microscopy system and method for checking a rotational position of a microscope camera

A microscopy system, comprising

• a microscope with a microscope camera and with an overview camera, wherein the microscope camera and the overview camera are two distinct cameras, wherein a rotational orientation of the overview camera relative to a sample stage of the microscope is known;
• and
• a computing device configured to:
• determine at least one image structure in an overview image captured with the overview camera suitable for use as an orientation determination feature in order to determine a rotational orientation of the microscope camera relative to the sample stage;
• instruct movement of the sample stage to bring the determined orientation determination feature closer to a center of a field of view of the microscope camera compared with a sample stage position used for capturing the overview image, and instruct capturing a microscope image of the orientation determination feature with the microscope camera;
• calculate a relative rotation between the microscope camera and the overview camera as a rotation between the orientation determination feature in the microscope image and the overview image;
• and
• calculate a rotational orientation of the microscope camera relative to the sample stage from the known rotational orientation of the overview camera relative to the sample stage and based on the calculated relative rotation between the microscope camera and the overview camera.