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US 12,590,965 B2Granted
Palmitoyl protein biomarkers in purified extracellular vesicles for early identification of clinically significant prostate cancer
AssigneesCedars-Sinai Medical Center, Vanderbilt University
InventorsDolores Di Vizio, Wei Yang, Javier Mariscal Avila, Tatyana Vagner, Sungyong You, Andries Zijlstra
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Abstract
The invention provides for methods for isolating large EVs and detecting palmitoyl proteins in the large EVs, as well as methods for detecting clinically significant prostate cancer based on the presence of palmitoyl proteins in the isolated large EVs in a subject in need thereof. The method further comprises administering cancer therapy to the subject.
Claims11
A method of detecting one or more palmitoyl proteins, comprising:
- obtaining a biological sample from a subject having prostate cancer or suspected of having prostate cancer;
- isolating large extracellular vesicles (EVs) from the biological sample;
- assaying the large EVs for palmitoyl proteins GNAQ, CD9, TLN1, STX11, ITGB3, LYN, SLC4A1, CANX, FLOT2, FLOT1, ABHD16A, MYADM, ATP2A3, TMX1, RAP2B, ATP2A2, SLC2A3, RTN4, LTBP1, and ABCC4;
- and
- detecting the palmitoyl proteins GNAQ, CD9, TLN1, STX11, ITGB3, LYN, SLC4A1, CANX, FLOT2, FLOT1, ABHD16A, MYADM, ATP2A3, TMX1, RAP2B, ATP2A2, SLC2A3, RTN4, LTBP1, and ABCC4.
2 more elements
A method of detecting clinically significant prostate cancer, comprising:
- obtaining a biological sample from a subject having prostate cancer or suspected of having prostate cancer;
- isolating large extracellular vesicles (EVs) from the biological sample;
- assaying the large EVs for palmitoyl proteins GNAQ, CD9, TLN1, STX11, ITGB3, LYN, SLC4A1, CANX, FLOT2, FLOT1, ABHD16A, MYADM, ATP2A3, TMX1, RAP2B, ATP2A2, SLC2A3, RTN4, LTBP1, and ABCC4;
- and
- detecting the palmitoyl proteins GNAQ, CD9, TLN1, STX11, ITGB3, LYN, SLC4A1, CANX, FLOT2, FLOT1, ABHD16A, MYADM, ATP2A3, TMX1, RAP2B, ATP2A2, SLC2A3, RTN4, LTBP1, and ABCC4,
- wherein the detection of the palmitoyl proteins indicates the presence of clinically significant prostate cancer.
3 more elements
Dependencies are inferred from the claim text.