Materials and methods for extracellular vesicle detection
AssigneeUniversity of Miami
InventorsSylvia Daunert, Yu-Ping Yang
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Abstract
Described herein is a method for detecting the presence of circulating extracellular vesicles in a subject. The method comprises contacting a biological sample from the subject with an antibody mimetic that specifically binds to a cell surface marker on the vesicles, wherein the antibody mimetic is coupled to a detectable label; and detecting presence of extracellular vesicles in the sample by detecting the presence of the detectable label coupled to the antibody mimetic bound to the vesicles.
Claims12
A method for detecting the presence of circulating extracellular vesicles in a subject, the method comprising
- (a) contacting a biological sample from the subject with a fusion protein comprising the amino acid sequence set forth in SEQ ID NO:
- 2, and wherein the fusion protein is coupled to a detectable label;
- (b) detecting presence of extracellular vesicles in the sample by detecting the presence of the detectable label coupled to the fusion protein bound to the vesicles.
A method of detecting the presence of circulating ovarian tumor-derived extracellular vesicles in a subject, the method comprising
- (a) contacting a biological sample from the subject with a fusion protein comprising the amino acid sequence set forth in SEQ ID NO:
- 2, and wherein the fusion protein is coupled to a detectable label;
- (b) detecting presence of ovarian tumor extracellular vesicles in the sample by detecting the presence of the detectable label coupled to the fusion protein bound to the ovarian tumor extracellular vesicles.
Dependencies are inferred from the claim text.