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US 12,590,959 B2Granted
Systems and methods for measuring cell signaling protein activity
AssigneeOctant, Inc.
InventorsHenry Chan, Aaron Cooper, Molly Jeanette Gasperini
Life of this patent
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Abstract
Described herein are systems for measuring enzymatic activities. Also described herein are methods for measuring or screening enzymatic activities utilizing the systems described herein.
Claims18
A system for measuring G protein-coupled receptor (GPCR) activity in a mammalian cell, the system comprising:
- a) a first exogenous effector of GPCR activity, wherein an expression of the first exogenous effector of GPCR activity is upregulated compared to an expression of the first exogenous effector of GPCR activity in a wild-type state, wherein the first exogenous effector of GPCR activity comprises a first adenylyl cyclase comprising ADCY6;
- b) a second exogenous effector of GPCR activity, wherein an expression of the second exogenous effector of GPCR activity is downregulated compared to an expression of the second exogenous effector of GPCR activity in a wild-type state, wherein the second exogenous effector of GPCR activity comprises a second adenylyl cyclase comprising ADCY3;
- and
- c) a reporter nucleic acid, wherein the reporter nucleic acid comprises a unique molecular identifier (UMI) nucleic acid sequence.
1 more element
Dependencies are inferred from the claim text.