Methods for screening cereblon modifying compounds
AssigneeCelgene Corporation
InventorsMary Matyskiela, Philip Chamberlain, Suzana Sturlini Couto, Katherine Stamp, Philip J. Sherratt, Gang Lu, Julia Hui
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Abstract
A method of screening a cereblon modifying compound for treating a disease or disorder, comprising: obtaining a sample; determining a first protein level of SALL4; administering the cereblon modifying compound to the sample; determining a second protein level of SALL4; comparing the first level and the second protein level of SALL4 to determine if the cereblon modifying compound induces degradation of SALL4; and selecting the cereblon modifying compound that does not induce degradation of SALL4.
Claims19
A method of screening a cereblon modifying compound for treating a disease or a disorder, comprising:
- (a) administering the cereblon modifying compound to a sample in vitro;
- (b) determining the ubiquitination level of Sal-like protein 4 (SALL4) in the sample;
- and
- (c) selecting the cereblon modifying compound if the ubiquitination level of SALL4 of (b) is non-detectable, or is reduced compared with the ubiquitination level of SALL4 in a control sample administered with a reference compound in vitro;
- wherein the reference compound is a cereblon modifying compound that induces ubiquitination of SALL4, and the sample and the control sample are from the same source.
A method of determining if a cereblon modifying compound can induce a teratogenic effect, comprising:
- (a) administering the cereblon modifying compound to a sample in vitro;
- and
- (b) determining the ubiquitination level of SALL4 in the sample,
- wherein if the ubiquitination level of SALL4 of (b) is non-detectable or is reduced compared with the ubiquitination level of SALL4 in a control sample administered with a reference compound in vitro, the cereblon modifying compound is determined to be non-teratogenic, or have a reduced teratogenic effect,
- wherein the reference compound is a cereblon modifying compound that induces ubiquitination of SALL4, and the sample and the control sample are from the same source.
Dependencies are inferred from the claim text.