Culture medium and culture method for primary cells of intestinal cancer

A culture medium for primary cells of intestinal cancer, comprising:

• an initial culture medium, Y27632, an antibiotic, gastrin, A8301, a non-essential amino acid, cholera toxin, insulin-like growth factor-1, nicotinamide, insulin, fetal bovine serum, and B27 additive,
• the initial culture medium is selected from the group consisting of Dulbecco's Modified Eagle Medium (DMEM), Ham's F-12 (F-12), DMEM/F12, and RPMI-1640, wherein,
• the concentration of Y27632 is 2.5-40 μM;
• the antibiotic is one or more selected from the group consisting of streptomycin/penicillin, amphotericin B and Primocin, in case of streptomycin/penicillin, with streptomycin having a concentration within the range of 25-400 μg/mL, with penicillin having a concentration within the range of 25-400 U/mL;
• in case of amphotericin B, having a concentration within the range of 0.25-4 μg/mL;
• and in case of Primocin, having a concentration within the range of 25-400 μg/mL;
• the concentration of gastrin is 1.25-20 nM;
• the concentration of A8301 is 25-200 nM;
• the non-essential amino acid is one or more selected from the group consisting of glycine, alanine, asparagine, aspartic acid, glutamic acid, proline and serine, with a total concentration of 25-400 μM;
• the concentration of cholera toxin is 1.25-20 ng/mL;
• the concentration of insulin-like growth factor-1 is 5-405 ng/ml;
• the concentration of nicotinamide is 2-8 mM;
• the concentration of insulin is 0.5-4 μg/mL;
• the volume concentration of fetal calf serum relative to the culture medium is 2.5% (v/v)-5% (v/v);
• and
• the volume ratio of the B27 additive to the culture medium is 1:25-1:200.