Method of introducing a sample into a separation column and corresponding system
AssigneeDionex Softron GmbH
InventorsChristoph Hollnagel, Hermann Hochgraeber, Thomas Wachinger
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Abstract
A method of introducing a sample into a separation column includes introducing the sample into a trap column, isolating the trap column from ambient atmosphere and pressurizing the trap column to a first pressure while the trap column is isolated from ambient atmosphere, providing a fluid connection between the trap column and the separation column after pressurizing the trap column to the first pressure, supplying the sample from the trap column to the separation column.
Claims18
A method of introducing a sample into a separation column of a liquid chromatography system, the method comprising:
- introducing the sample into a trap column;
- isolating the trap column from an ambient atmosphere and pressurizing the trap column to a first pressure while the trap column is isolated from the ambient atmosphere;
- providing a fluid connection between the trap column and the separation column after pressurizing the trap column to the first pressure;
- and
- supplying the sample from the trap column to the separation column, wherein the liquid chromatography system comprises an analytical pump adapted to provide a flow of pressurized fluid, wherein the method further comprises:
- providing a fluid connection between the trap column and the analytical pump, wherein the fluid connection between the trap column and the analytical pump is provided after pressurizing the trap column to the first pressure.
Dependencies are inferred from the claim text.