Cell sorting chip, device and method based on dielectrophoresis induced deterministic lateral displacement

A cell sorting microfluidic chip based on dielectrophoresis induced deterministic lateral displacement, comprising a micro-channel, wherein the micro-channel comprises a sample inlet, a straight channel and two sample outlets;

• three groups of electrode array pairs are integrated at a bottom of the straight channel, the three groups of electrode array pairs respectively being a focusing electrode group, a sorting electrode group and a separation electrode group;
• the focusing electrode group is used for cell focusing and signal detection;
• the sorting electrode group is used for single cell sorting;
• and the separation electrode group is used for single cell separation;
• wherein:
• the focusing electrode group comprises one or more electrode pairs, the separation electrode group comprises one or more electrode pairs, and the sorting electrode group comprises at least one electrode;
• each electrode in the one or more electrode pairs of the focusing electrode group has two first arms connected at a first tip point, a first centerline of the focusing electrode group passes the first tip point of each electrode in the one or more electrode pairs, an included angle between one of the two first arms and the first centerline has a value a, an included angle between the other one of the two first arms and the first centerline has a value b, an included angle between the two first arms of each electrode has a value a+b;
• the two first arms of different electrodes in the one or more electrode pairs of the focusing electrode group are parallel to each other, relatively, and the electrodes in the one or more electrode pairs of the focusing electrode group are distributed in a spaced manner;
• each electrode in the one or more electrode pairs of the separation electrode group has two second arms connected at a second tip point, a second centerline of the separation electrode group passes the second tip point of each electrode in the one or more electrode pairs, an included angle between one of the two second arms and the second centerline has a value a, an included angle between the other one of the two second arms and the second centerline has a value b, an included angle between the two second arms of each electrode has a value a+b;
• each electrode in the one or more electrode pairs of the separation electrode group and each electrode in the one or more electrode pairs of the focusing electrode group have a same structure, while the included angle between the two first arms of each electrode in the one or more electrode pairs of the focusing electrode group and the included angle between the two second arms of each electrode in the one or more electrode pairs of the separation electrode group are of different directions;
• and
• the at least one electrode of the sorting electrode group has a third arm, and an included angle between the third arm and the first centerline of the focusing electrode group has a value a.