Method and device for detection of ampicillin-resistant non-typhoidal Salmonella
AssigneeTaipei Medical University
InventorsShiuh-Bin Fang, Wei-Chiao Chang, Wan-Hsuan Chou, Ke-Chuan Wang
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Abstract
Disclosed is a method and a device for determining whether a sample contains ampicillin-resistant (AMP-R) non-typhoid Salmonella (NTS). The method comprises detecting, in the sample, the presence of a combination of certain genes or their gene products, wherein the presence of said combination indicates the sample contains AMP-R NTS.
Claims10
A method for detecting ampicillin-resistant (AMP-R) non-typhoidal Salmonella (NTS) in a sample from a patient, comprising
- (a) collecting the sample from the patient;
- (b) extracting DNA from the sample;
- (c) performing a polymerase chain reaction (PCR) using the extracted DNA to detect, in the sample, the presence of a combination of a plurality of genes wherein the plurality of genes—consist of a blaTEM-1b gene, a blaCARB-2 gene, and optionally one or more genes selected from the group consisting of a blaCMY-2 gene, blaOXA-1 gene and an ampC gene, wherein the performing a PCR produces amplified copies of each gene of the combination of said plurality of genes and/or amplified fragments of each gene of the combination of said plurality of genes, thereby detecting AMP-R NTS in said sample.
A method for detecting a combination of genes from ampicillin-resistant (AMP-R) non-typhoidal Salmonella (NTS) in a sample from a patient, comprising
- (a) collecting the sample from the patient;
- (b) extracting DNA from the sample;
- (c) performing a polymerase chain reaction (PCR) using the extracted DNA to detect, in the sample, the presence of nucleic acids from a combination of genes, wherein the combination of genes consists of a blaTEM-1b gene, a blaCARB-2 gene, and optionally one or more genes selected from the group consisting of a blaCMY-2 gene, and an ampC gene, wherein the performing a PCR produces amplified copies of each gene of the combination of said plurality of genes, and/or amplified fragments of each gene of the combination of genes, thereby detecting AMP-R NTS in said sample.
A method for treating a patient having an ampicillin-resistant non-typhoidal Salmonella infection, which comprises detecting a combination of genes from ampicillin-resistant (AMP-R) non-typhoidal Salmonella (NTS) in a sample from the patient, comprising
- (a) collecting the sample from the patient;
- (b) extracting DNA from the sample;
- (c) performing a polymerase chain reaction (PCR) using the extracted DNA to detect, in the sample, the presence of a combination of genes, wherein the combination of genes consists of a blaTEM-1b gene, a blaCARB-2 gene, and optionally one or more genes selected from the group consisting of a blaCMY-2 gene, and an ampC gene;
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- (d) administering an antibiotic other than ampicillin to the patient from whom said sample was collected in which said combination of genes were detected.
Dependencies are inferred from the claim text.