Back to results
US 12,590,334 B2Granted
Methods for nucleic acid sequence detection
AssigneeBioSpyder Technologies, Inc.
InventorsMilos Babic, Christy Lee Trejo, Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann
Life of this patent
Estimating the term…
Abstract
Barcoded ligation assay products from individual samples.
Claims28
A method for detecting target nucleic acid sequences in samples, wherein a target sequence has a downstream region (DR) and an upstream region (UR), comprising
- (a) contacting the samples with a pair of detector oligos (DOs), which pair comprises
- a downstream detector oligo (DDO) having a complementary downstream region (DR′) and
- a separate upstream detector oligo (UDO) having a complementary upstream region (UR′),
- wherein at least one of the DDO or UDO has a second complementary region (DR2′ or UR2′) separated from the DR′ or UR′ by a noncomplementary region (CP1) that does not hybridize to the target nucleic acid, whereby the DR2′ or UR2′ specifically hybridizes to a DR2 or UR2 of the target nucleic acid,
- and where at least one of the DDO or UDO has been labeled with a barcode sequence,
- thereby allowing the pair of detectors to hybridize specifically to target nucleic acids;
- (b) whereby the DR′ and UR′ are ligated if both are specifically hybridized to the DR and UR of a target sequence;
- and
- (c) optionally labeling the ligated detectors with a barcode sequence in a plurality of samples;
- whereby the barcoded ligation product indicates the presence of the target sequence and identifies the sample.
7 more elements
A method for detecting target nucleic acid sequences in samples, wherein a target sequence has a downstream region (DR) and an upstream region (UR), comprising
- (a) contacting the samples with a pair of detector oligos (DOs), which pair comprises
- a downstream detector oligo (DDO) having a complementary downstream region (DR′) and
- a separate upstream detector oligo (UDO) having a complementary upstream region (UR′),
- wherein at least one of the DDO or UDO has a second complementary region (DR2′ or UR2′) separated from the DR′ or UR′ by a noncomplementary region (CP1) that does not hybridize to the target nucleic acid, whereby the DR2′ or UR2′ specifically hybridizes to a DR2 or UR2 of the target nucleic acid,
- and where at least one of the DDO or UDO contains a barcode sequence,
- thereby allowing the pair of detectors to hybridize specifically to target nucleic acids;
- (b) whereby the DR′ and UR′ are ligated if both are specifically hybridized to the DR and UR of a target sequence;
- and
- (c) optionally labeling the ligated detectors with a barcode sequence in a plurality of samples;
- whereby the barcoded ligation product indicates the presence of the target sequence and identifies the sample.
7 more elements
Dependencies are inferred from the claim text.