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AssigneeInstitute of Oil Crops Chinese Academy of Agricultural Sciences
InventorsLijiang Liu, Shengyi Liu, Yi Zhang, Yupo Wu, Chuanji Zhao, Xiaohui Cheng, Fan Liu, Li Ren, Junyan Huang, Xiaoling Dun
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The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.
A method for obtaining a Brassica napus plant resistant to clubroot disease, comprising the step of knocking out the GSL5 gene in the plant, wherein the knocking out results in the loss of function of the gene, wherein the GSL5 gene is located in subgenome A of the Brassica napus and has the nucleotide sequence set forth in SEQ ID NO:
A method for obtaining a Brassica napus plant resistant to clubroot disease, comprising the step of knocking out the GSL5 gene in the plant, wherein the knocking out results in the loss of function of the gene, wherein the GSL5 gene is located in subgenome C of the Brassica napus and has the nucleotide sequence set forth in SEQ ID NO:
A method for obtaining a Brassica rapa plant resistant to clubroot disease, comprising the step of knocking out the GSL5 gene in the plant, wherein the knocking out results in the loss of function of the gene and wherein the GSL5 gene in the Brassica rapa has the nucleotide sequence set forth in SEQ ID NO:
A method for obtaining a Brassica oleracea plant resistant to clubroot disease, comprising the step of knocking out the GSL5 gene in the plant, wherein the knocking out results in the loss of function of the gene and wherein the GSL5 gene in the Brassica oleracea has the nucleotide sequence set forth in SEQ ID NO:
Dependencies are inferred from the claim text.