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US 12,590,315 B2Granted
Compositions and methods based on QPT engineering for producing tobacco plants and products having altered alkaloid levels
AssigneeAltria Client Services LLC
InventorsDong Qi, Chengalrayan Kudithipudi, Yanxin Shen
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Abstract
The present disclosure provides compositions and methods related to tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.
Claims19
A tobacco plant, or part thereof, comprising:
- (a) a first non-naturally occurring mutant allele of an endogenous QUINOLINATE PHOSPHORIBOSYL TRANSFERASE (QPT) 2a gene, the endogenous OPT2a gene having the nucleic acid sequence of SEQ ID NO:
- 7, wherein the first non-naturally occurring mutant allele comprises a first deletion as compared to the endogenous OPT2a gene, and wherein the first deletion either a deletion of nucleotide positions 264 to 274 as compared to the nucleic acid sequence of SEQ ID NO:
- 7 or a deletion of nucleotide positions 268 to 274 as compared to the nucleic acid sequence of SEQ ID NO:
- 7, and
- (b) a second non-naturally occurring mutant allele of an endogenous QPT2b gene, the endogenous OPT2b gene having the nucleic acid sequence of SEQ ID NO:
- 8, wherein the second non-naturally occurring mutant allele comprises a second deletion as compared to the endogenous OPT2b gene, and wherein the second deletion consists of a deletion of nucleotide positions 269 to 276 as compared to the nucleic acid sequence of SEQ ID NO:
- 8.
4 more elements
Dependencies are inferred from the claim text.