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US 12,590,313 B2Granted
Methodologies and compositions for creating targeted recombination and breaking linkage between traits
AssigneeCorteva Agriscience LLC
InventorsSandeep Kumar, Ryan M. Lee, Stephen Novak, Andrew F. Worden
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Abstract
The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.
Claims24
A method for increasing the frequency of genetic recombination between a first locus genetically linked to a second locus within a genome of a plant, the method comprising the steps of:
- a) introducing a site specific nuclease into the genome of the plant;
- b) producing a double stranded break at a genomic target site with the site specific nuclease in one of two homologous chromosomes, wherein the double stranded break is produced by a site specific nuclease in one of two homologous chromosomes, and a double stranded break is not produced in the second homologous chromosome;
- c) undergoing recombination within the plant genome;
- and,
- d) modifying the plant genome, wherein the modified plant genome comprises increased frequency of genetic recombination between the first locus and the second locus to reduce the genetic linkage between the first locus and the second locus.
2 more elements
Dependencies are inferred from the claim text.