Screening of fluorescent microbes using microfabricated device
AssigneeIsolation Bio Inc.
InventorCrystal Emery
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Abstract
A method of identifying a fluorescent cell in a sample using a microfabricated chip having a top surface including a plurality of microwells. At least one cell of a sample is loaded into at least one microwell of the plurality of microwells. The microfabricated chip is incubated to grow a population of cells from the at least one cell in the at least one microwell. Fluorescence exhibited by at least one microwell is detected by analyzing an image of the microfabricated chip, to thereby determine a presence of a fluorescent cell of interest in the sample.
Claims6
A method of identifying a fluorescent live cell using a microfabricated chip having a top surface including a plurality of microwells, the method comprising:
- (a) loading at least one cell of a sample into at least one microwell of the plurality of microwells, wherein the at least one microwell is also loaded with a metabolic indicator which is a fluorescent dye indicating the metabolic activity of cells;
- (b) incubating the microfabricated chip to grow a population of cells from the at least one cell in the at least one microwell;
- and
- (c) detecting fluorescence exhibited by at least one microwell by analyzing an image of the microfabricated chip, to thereby determine a presence of a fluorescent live cell of interest in the sample,
- wherein the detecting comprises detecting fluorescence of the metabolic indicator and fluorescence of the population of cells simultaneously at separate fluorescence detection channels.
A method of identifying a fluorescent live cell in a sample using a microfabricated chip having a top surface including a plurality of microwells, the method comprising:
- (a) loading at least one cell of a sample and a fluorescent metabolic indicator being a fluorescent dye into at least one microwell of the plurality of microwells, the fluorescence status of the metabolic indicator indicating presence or absence of cell metabolic activity;
- (b) incubating the microfabricated chip to grow a population of cells from the at least one cell in the at least one microwell;
- (c) detecting fluorescence exhibited by at least one microwell by analyzing an image of the microfabricated chip, to thereby determine a presence of a fluorescent live cell of interest in the sample,
- wherein detecting fluorescence exhibited by at least one microwell comprises detecting fluorescence of the metabolic indicator and fluorescence of the population of cells simultaneously at separate fluorescence detection channels.
Dependencies are inferred from the claim text.