Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
AssigneeThe General Hospital Corporation
InventorsJ. Keith Joung, Benjamin Kleinstiver
Life of this patent
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Abstract
Engineered CRISPR from Prevotella and Francisella 1 (Cpf1) nucleases with altered and improved target specificity and their use in genomic engineering, epigenomic engineering, genome targeting, genome editing, and in vitro diagnostics.
Claims33
An isolated CRISPR from Prevotella and Francisella 1 (Cpf1) protein, wherein the protein is from Lachnospiraceae bacterium ND2006 (LbCpf1), comprising a sequence that is at least 80% identical to the amino acid sequence of amino acids 1-1228 of SEQ ID NO:
- 10, with mutations at one or more of the following positions:
- N160, S168, N256, N260, K272, S286, K349, D505, Q513, G741, Q944, K945, and/or S985 of amino acids 1-1228 of SEQ ID NO:10.
An isolated nucleic acid encoding an isolated Cpf1 protein, wherein the protein is from LbCpf1, comprising a sequence that is at least 80% identical to the amino acid sequence of amino acids 1-1228 of SEQ ID NO:
- 10, with mutations at one or more of the following positions:
- N160, S168, N256, N260, K272, S286, K349, D505, Q513, G741, Q944, K945, and/or S985 of amino acids 1-1228 of SEQ ID NO:10.
Dependencies are inferred from the claim text.