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US 12,590,298 B2Granted
Ex vivo tumor angiogenesis model
AssigneeThe Regents of the University of California
InventorsHui Sun, Adrian Chichuen Au, Guo Cheng
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Abstract
The present disclosure relates generally to ex vivo primary tumor models prepared from fresh tumor tissues which are useful for screening anti-cancer agents. The fresh tumor tissues are prepared and cultured under suitable conditions to grow an outgrowth of endothelial cells. Killing of these endothelial cells by a candidate agent indicates the efficacy of the agent in inhibiting tumor angiogenesis.
Claims14
A method for growing endothelial cells around a tumor tissue, comprising:
- embedding a tumor tissue in a biological matrix, wherein the tumor tissue has a diameter between 0.1 mm and 1.5 mm, is substantially homogeneous, and is substantially free of fibrosis, necrosis or hemorrhage, and wherein the biological matrix comprises at least an angiogenic factor selected from VEGF, FGF, EGF and angiopoietin;
- and
- culturing the tumor tissue in the biological matrix under conditions to promote growth of endothelial cells from the tumor tissue, for a time sufficient such that the tumor tissue grows into a tumor model comprising at least one endothelial cell outgrowth surrounding a tumor core, and wherein the tumor model has a diameter of at least 3 mm.
Dependencies are inferred from the claim text.