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US 12,590,294 B2Granted
Process for producing cardiomyocytes
AssigneeOxford University Innovation Limited
InventorsMing Lei, Derek Terrar, Faizzan Ahmad
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Abstract
The present invention relates to a process for producing a population of cells which comprises mature atrial cardiomyocytes. The process comprises the step of treating iPS cells according to a treatment regimen which comprises contacting the iPS cells with Gremlin2 and retinoic acid, such that at least a portion of the iPS cells differentiate into mature atrial cardiomyocytes.
Claims11
A process for producing a population of cells which comprises mature atrial cardiomyocytes, the process comprising the step:
- (a) treating a first population of cells according to a treatment regimen, wherein the first population of cells comprises iPS cells, and wherein the treatment regimen comprises:
- (i) culturing the first population of cells in a first cardiac cell differentiation medium which comprises a moiety which is capable of potentiating the WNT signalling pathway between Day 0 and Day 2;
- (ii) culturing the first population of cells in a second cardiac cell differentiation medium which comprises a moiety which is capable of attenuating the WNT signalling pathway between Day 2 and Day 4;
- (iii) culturing the first population of cells in a third cardiac cell differentiation medium comprising Gremlin2 between Day 4 and Day 6;
- and then
- (iv) culturing the first population of cells in a fourth cardiac cell differentiation medium comprising retinoic acid between Day 6 and Day 10;
- such that at least a portion of the iPS cells in the first population of cells differentiate into mature atrial cardiomyocytes, thereby producing a second population of cells which comprises mature atrial cardiomyocytes.
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