Method for obtaining a platelet derived secretome and uses thereof

An in vitro method for the production of a platelet derived secretome characterized by comprising the following steps:

• a) obtaining a non-activated platelet suspension free of plasma and other blood/tissue components other than platelets by the steps of:
• i) centrifugation and/or filtration of a sample previously obtained from a biological source comprising platelets or from a platelet rich sample;
• ii) isolation of the platelet fraction;
• and
• iii) resuspension of the platelet fraction in a buffer;
• wherein step a) is performed at room temperature, and wherein step a) does not use inhibitors of platelet aggregation;
• b) inducing platelet degranulation by incubating in constant shaking the non-activated platelet suspension obtained in step a) with a platelet agonist, obtaining a platelet and platelet secretome suspension;
• wherein the temperature of step b) is between 35 and 40° C.;
• and
• c) collecting the platelet derived secretome by centrifuging the platelet and platelet secretome suspension obtained in step b),
• wherein step c) is performed at room temperature.