Method of inducing expression of calcium channel and/or calcium pump, and apparatus therefor
AssigneeNichia Corporation
InventorsShinpei Kawarai, Atsushi Tsukamoto, Yasuo Fujikawa, Tomohiro Tsurumoto
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Abstract
A method of inducing expression of a calcium channel and/or a calcium pump in a cell includes: irradiating the cell with light in a wavelength range of 315-325 nm. The calcium channel and/or the calcium pump is/are at least one selected from the group consisting of dihydropyridine receptor (DHPR), voltage-gated calcium channel (VGCC), ryanodine receptor (RYR), and sarcoendoplasmic reticulum Ca2+-ATPase (SERCA).
Claims10
A method of inducing gene expression of a calcium channel and/or a calcium pump in a cell, the method comprising:
- irradiating the cell with light in a wavelength range of 315-325 nm,
- wherein the calcium channel and/or the calcium pump is/are at least one selected from the group consisting of dihydropyridine receptor (DHPR), voltage-gated calcium channel (VGCC), ryanodine receptor (RYR), and sarcoendoplasmic reticulum Ca2+-ATPase (SERCA).
A method of making a cell having induced gene expression of a calcium channel and/or a calcium pump, comprising:
- irradiating a cell with light in a wavelength range of 315-325 nm;
- and
- freezing the irradiated cell,
- wherein, in the step of irradiating:
- the cell is not irradiated with light at any wavelength of 200-300 nm, or is irradiated with light in wavelengths of 200-300 nm at a fluence that is less than 30% of that of the light in the wavelength range of 315-325 nm, and
- the cell is not irradiated with light at any wavelength of 330-400 nm, or is irradiated with light in wavelengths of 330-400 nm at a fluence that is less than 50% of that of the light in the wavelength range of 315-325 nm.
Dependencies are inferred from the claim text.