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US 12,590,290 B2Granted
Method for treating and modelling hearing loss
AssigneeMackay Medical College
InventorYi-Chao Hsu
Life of this patent
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Abstract
A method for treating hearing loss in a subject in need thereof is provided. The method includes providing a combination of transcription factors to induce generation of a hair cell-like cell, thereby treating the hearing loss in the subject. The generated hair cell-like cells exhibit characteristic of mature functional cells that is useful in cell replacement therapy for autologous transplantation.
Claims13
A method for treating hearing loss in a subject in need thereof, comprising:
- providing a set of nucleic acids or a set of polypeptides;
- inducing generation of a mature hair cell-like cell by culturing an otic progenitor cell in the presence of the set of nucleic acids or the set of polypeptides;
- and
- delivering the mature hair cell-like cell into an inner ear of the subject,
- wherein the set of nucleic acids comprises a first nucleic acid encoding ATOH1, a second nucleic acid encoding RFX1, and a third nucleic acid encoding RFX3, or wherein the set of polypeptides comprises ATOH1, RFX1, and RFX3.
2 more elements
A method of generating a mature hair cell-like cell from a pluripotent stem cell in vitro, comprising:
- differentiating the pluripotent stem cell into an otic progenitor cell;
- and
- culturing the otic progenitor cell in the presence of a set of nucleic acids or a set of polypeptides to obtain a population comprising the mature hair cell-like cell,
- wherein the set of nucleic acids comprises a first nucleic acid encoding ATOH1, a second nucleic acid encoding RFX1, and a third nucleic acid encoding RFX3, and wherein the set of polypeptides comprises ATOH1, RFX1, and RFX3.
1 more element
Dependencies are inferred from the claim text.