Medium-based method realized for differentiation of dental stem cells into neurons

A medium-based method realized for a differentiation of dental stem cells into neurons, wherein the medium-based method enables mesenchymal stem cells obtained from a dental tissue to be differentiated into dopaminergic neurons, and the medium-based method comprises:

• seeding the dental stem cells at a concentration of 5000 cells/cm2,
• following a 24-hour incubation, introducing the dental stem cells into a first part neurogenic induction medium and continuing a first medium application for 4 days,
• subsequently, introducing the dental stem cells into a second part neurogenic induction medium and continuing a second medium application for 2 days, and
• terminating the differentiation at an end of 6 days, wherein
• the first part neurogenic induction medium comprises:
• Dulbecco's Modified Eagle Medium/Ham's F-12 (DMEM/F12) medium supplemented with L-alanyl-L-glutamine,
• a serum-free neuronal supplement at 1%,
• 3-isobutyl-1-methylxanthine (IBMX) 100 μM,
• a valproic acid sodium salt (VPA) 2 mM,
• forskolin 0.1 μM,
• basic fibroblast growth factor (bFGF) 20 ng/ml, and
• epidermal growth factor (EGF) 20 ng/ml;
• and
• the second part neurogenic induction medium comprises:
• DMEM/F12 medium supplemented with L-alanyl-L-glutamine,
• a serum-free neuronal supplement at 1%,
• IBMX 100 μM,
• VPA 2 mM,
• forskolin 0.1 μM,
• bFGF 20 ng/ml,
• EGF 20 ng/ml, and
• a brain-derived neurotrophic factor 30 ng/ml.